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- W2275206142 abstract "The CP4-EPSPS gene is widely used in herbicide-tolerant plants/crops all over the world.In this study,a method was developed by coupling liquid chromatography with high sensitivity to tandem mass spectrometry to quantify the amount of CP4-EPSPS expression in Nicotiana tabacum leaves.The quantification of protein was converted to measure the unique peptide of CP4-EPSPS protein.One peptide unique to CP4-EPSPS was synthesized and labeled with H18 8 2O to get 1O stable isotope labeled peptide.The peptide served as the internal standard.The validated method had good specificity and linearity.The intra-and inter-day precisions and accuracy for all samples were satisfactory.The results demonstrated that the novel method was sensitive and selective to quantify CP4-EPSPS in the crude extract without time-consuming pre-separation or the purification procedures." @default.
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- W2275206142 date "2015-01-01" @default.
- W2275206142 modified "2023-09-24" @default.
- W2275206142 title "Quantification of CP4-EPSPS in genetically modified Nicotiana tabacum leaves by LC-MS/MS with 18O-labeling" @default.
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