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• W2275220963 abstract "Cows infected with Escherichia (E.) coli usually experience severe clinical symptoms, including damage to mammary tissues, reduced milk yield, and altered milk composition. In order to investigate the host response to E. coli infection and discover novel markers for mastitis treatment, mammary tissue samples were collected from healthy cows and bovines with naturally occurring severe E. coli mastitis. Changes of mammary tissue proteins were examined using two-dimensional gel electrophoresis and label-free proteomic approaches. A total of 95 differentially expressed proteins were identified. Of these, 56 proteins were categorized according to molecular function, cellular component, and biological processes. The most frequent biological processes influenced by the proteins were response to stress, transport, and establishment of localization. Furthermore, a network analysis of the proteins with altered expression in mammary tissues demonstrated that these factors are predominantly involved with binding and structural molecule activities. Vimentin and a-enolase were central functional hubs in the network. Based on results from the present study, disease-induced alterations of protein expression in mammary glands and potential markers for the effective treatment of E. coli mastitis were identified. These data have also helped elucidate defense mechanisms that protect the mammary glands and promote the pathogenesis of E. coli mastitis." @default.
• W2275220963 created "2016-06-24" @default.
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• W2275220963 date "2015-01-01" @default.
• W2275220963 modified "2023-10-17" @default.
• W2275220963 title "Comparative proteomic analysis of proteins expression changes in the mammary tissue of cows infected with<i>Escherichia coli</i>mastitis" @default.
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• W2275220963 doi "https://doi.org/10.4142/jvs.2015.16.3.253" @default.
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