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- W2275242227 abstract "Increasing the volumetric imaging speed of light-sheet microscopy will improve its ability to detect fast changes in neural activity. Here, a system is introduced for brain-wide imaging of neural activity in the larval zebrafish by coupling structured illumination with cubic phase extended depth-of-field (EDoF) pupil encoding. This microscope enables faster light-sheet imaging and facilitates arbitrary plane scanning-removing constraints on acquisition speed, alignment tolerances, and physical motion near the sample. The usefulness of this method is demonstrated by performing multi-plane calcium imaging in the fish brain with a 416×832×160 μm field of view at 33 Hz. The optomotor response behavior of the zebrafish is monitored at high speeds, and time-locked correlations of neuronal activity are resolved across its brain." @default.
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- W2275242227 date "2016-02-17" @default.
- W2275242227 modified "2023-10-17" @default.
- W2275242227 title "Calcium imaging of neural circuits with extended depth-of-field light-sheet microscopy" @default.
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- W2275242227 doi "https://doi.org/10.1364/ol.41.000855" @default.
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