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- W227524805 abstract "The authors have described some of the properties of a simple coulometric enzyme amplified detection method and its application to enzyme immunoassay. This principle has been illustrated with an alkaline phosphatase enzyme label giving rise to NAD + , the catalytic activator for a specific redox cycle which uses the ferricyanide ion as the oxidant. The important features of this system are: high specificty of the redox cycle for NAD + compared with NADP + ; a high turnover number for the alkaline phosphatase/NADP + combination; and a high V max /K m ratio of the alcohol dehydrogenase and diaphorase for NAD + and NADH" @default.
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- W227524805 date "1989-01-01" @default.
- W227524805 modified "2023-09-26" @default.
- W227524805 title "An electrochemical immunoassay for prostatic acid phosphatase incorporating enzyme amplification" @default.
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