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- W2275265554 abstract "Gene therapy remains one of the potential strategies to achieve a cure for HIV infection. One of the major limitations of anti-HIV gene therapy concerns recovering an adequate number of modified cells to generate an HIV-proof immune system. Our study addresses this issue by developing a methodology that can mark conditional vector-transformed cells for selection and subsequently target HIV-infected cells for elimination by treatment with ganciclovir (GCV). We used the herpes simplex virus thymidine kinase (TK) mutant SR39, which is highly potent at killing cells at low GCV concentrations. This gene was cloned into a conditional HIV vector, pNL-GFPRRESA, which expresses the gene of interest as well as green fluorescent protein (GFP) in the presence of HIV Tat protein. We show here that TK-SR39 was more potent that wild-type TK (TK-WT) at eliminating infected cells at lower concentrations of GCV. As the vector expresses GFP in the presence of Tat, transient expression of Tat either by Tat RNA transfection or transduction by a nonintegrating lentiviral (NIL) vector marked the cells with GFP for selection. In cells selected by this strategy, TK-SR39 was more potent at limiting virus replication than TK-WT. Finally, in Jurkat cells modified and selected by this approach, infection with CXCR4-tropic Lai virus could be suppressed by treatment with GCV. GCV treatment limited the number of HIV-infected cells, virus production, as well as virus-induced cytopathic effects in this model. We provide proof of principle that TK-SR39 in a conditional HIV vector can provide a safe and effective anti-HIV strategy." @default.
- W2275265554 created "2016-06-24" @default.
- W2275265554 creator A5012620834 @default.
- W2275265554 creator A5091809007 @default.
- W2275265554 date "2016-05-01" @default.
- W2275265554 modified "2023-09-25" @default.
- W2275265554 title "Conditional Cytotoxic Anti-HIV Gene Therapy for Selectable Cell Modification" @default.
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- W2275265554 cites W1980424217 @default.
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- W2275265554 cites W1984843257 @default.
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- W2275265554 cites W1988272801 @default.
- W2275265554 cites W1989883593 @default.
- W2275265554 cites W1990170697 @default.
- W2275265554 cites W1990439902 @default.
- W2275265554 cites W1990466785 @default.
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- W2275265554 cites W1991696977 @default.
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- W2275265554 doi "https://doi.org/10.1089/hum.2015.126" @default.
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- W2275265554 hasPublicationYear "2016" @default.
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