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- W2275290717 startingPage "2527" @default.
- W2275290717 abstract "Reconstitution of Z-disks extracted with a low ionic strength solution by incubating proteins released from myofibrils by Ca2+ -activated factor (CAF) with Z-disk-extracted fiber bundles was successful, but the physiological activity of the fiber bundles with reconstituted Z-disks has not been studied. To elucidate the relationships between the structure of Z-disks and physiological activity of myofibrils, the contractilities of intact, Z-disk-extracted and -reconstituted fiber bundles were studied by optical and electron microscopy. Intact fiber bundles that contracted immediately after the addition of contracting solution (Mg2+ -ATP solution) showed the disappearance of the I-band and the formation of a double overlap of thin filaments in the center of the A-band. The contractility of the fiber bundles decreased with the progress of extraction of Z-disks, and formation of the double overlap of thin filaments in the center of the A-band was not observed. The fiber bundles with Z-disks reconstituted recovered the mechanism of contraction similar to that of the intact fiber bundles. When the Z-disks were removed by CAF instead of the low ionic strength solution, the reconstitution of Z-disks was not successful and therefore the contractility of the fiber bundles was not recovered." @default.
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- W2275290717 date "1981-01-01" @default.
- W2275290717 modified "2023-09-27" @default.
- W2275290717 title "Studies on reconstitution of Z-disks. Contractility of myofibrils with reconstituted Z-disks." @default.
- W2275290717 doi "https://doi.org/10.1271/bbb1961.45.2527" @default.
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