Matches in SemOpenAlex for { <https://semopenalex.org/work/W2275396013> ?p ?o ?g. }
Showing items 1 to 62 of
62
with 100 items per page.
- W2275396013 endingPage "R1175" @default.
- W2275396013 startingPage "R1168" @default.
- W2275396013 abstract "6-Phosphofructo-2-kinase (PFK-2) was analyzed in four organs of the anoxia-tolerant marine gastropod mollusk Busycon canaliculatum. Whelk PFK-2 resembled the nonhepatic enzyme from mammals with highest activity occurring in gill (22 pmol.min-1.g-1). Hepatopancreas PFK-2 was purified over 8,000-fold to a final specific activity of 11 mU/mg protein (at 20 degrees C) and gave a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme was a dimer with a native molecular mass of 142 kDa and a subunit molecular mass of 67 kDa. The purified enzyme showed negligible fructose-2,6-bisphosphatase (FBPase-2) activity, although the activity ratio of PFK-2 to FBPase-2 was 0.625 in crude extracts. In response to environmental anoxia, the activity of PFK-2 dropped in all organs to 34-56% of the corresponding aerobic value (half-time was 2 h in gill), and the Michaelis constant for fructose 6-phosphate increased by 50% (to 92 microM in gill). These changes paralleled decreases in organ fructose 2,6-bisphosphate concentration and pyruvate kinase activity and contribute to the overall glycolytic rate depression induced by anoxia in this facultative anaerobe. In vitro treatment of the anoxic form of hepatopancreas PFK-2 with alkaline phosphatase increased enzyme activity, suggesting that the aerobic and anoxic enzyme forms are interconverted by reversible protein phosphorylation. However, the protein kinase involved in this process is not yet known; incubation of aerobic PFK-2 with Mg-ATP plus adenosine 3',5'-cyclic monophosphate-dependent protein kinase or protein kinase C did not alter enzyme activity." @default.
- W2275396013 created "2016-06-24" @default.
- W2275396013 creator A5031573079 @default.
- W2275396013 creator A5080423361 @default.
- W2275396013 date "1991-06-01" @default.
- W2275396013 modified "2023-10-03" @default.
- W2275396013 title "Inactivation of 6-phosphofructo-2-kinase during anaerobiosis in the marine whelk Busycon canaliculatum" @default.
- W2275396013 doi "https://doi.org/10.1152/ajpregu.1991.260.6.r1168" @default.
- W2275396013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1647700" @default.
- W2275396013 hasPublicationYear "1991" @default.
- W2275396013 type Work @default.
- W2275396013 sameAs 2275396013 @default.
- W2275396013 citedByCount "2" @default.
- W2275396013 crossrefType "journal-article" @default.
- W2275396013 hasAuthorship W2275396013A5031573079 @default.
- W2275396013 hasAuthorship W2275396013A5080423361 @default.
- W2275396013 hasConcept C104292427 @default.
- W2275396013 hasConcept C104317684 @default.
- W2275396013 hasConcept C122245378 @default.
- W2275396013 hasConcept C181199279 @default.
- W2275396013 hasConcept C185592680 @default.
- W2275396013 hasConcept C20251656 @default.
- W2275396013 hasConcept C2776970464 @default.
- W2275396013 hasConcept C2780854706 @default.
- W2275396013 hasConcept C28212737 @default.
- W2275396013 hasConcept C55493867 @default.
- W2275396013 hasConcept C58431802 @default.
- W2275396013 hasConcept C86803240 @default.
- W2275396013 hasConceptScore W2275396013C104292427 @default.
- W2275396013 hasConceptScore W2275396013C104317684 @default.
- W2275396013 hasConceptScore W2275396013C122245378 @default.
- W2275396013 hasConceptScore W2275396013C181199279 @default.
- W2275396013 hasConceptScore W2275396013C185592680 @default.
- W2275396013 hasConceptScore W2275396013C20251656 @default.
- W2275396013 hasConceptScore W2275396013C2776970464 @default.
- W2275396013 hasConceptScore W2275396013C2780854706 @default.
- W2275396013 hasConceptScore W2275396013C28212737 @default.
- W2275396013 hasConceptScore W2275396013C55493867 @default.
- W2275396013 hasConceptScore W2275396013C58431802 @default.
- W2275396013 hasConceptScore W2275396013C86803240 @default.
- W2275396013 hasIssue "6" @default.
- W2275396013 hasLocation W22753960131 @default.
- W2275396013 hasLocation W22753960132 @default.
- W2275396013 hasOpenAccess W2275396013 @default.
- W2275396013 hasPrimaryLocation W22753960131 @default.
- W2275396013 hasRelatedWork W1994303044 @default.
- W2275396013 hasRelatedWork W2008102720 @default.
- W2275396013 hasRelatedWork W2042097357 @default.
- W2275396013 hasRelatedWork W2088399868 @default.
- W2275396013 hasRelatedWork W2165167785 @default.
- W2275396013 hasRelatedWork W2426963118 @default.
- W2275396013 hasRelatedWork W2464931466 @default.
- W2275396013 hasRelatedWork W4211064073 @default.
- W2275396013 hasRelatedWork W4242612276 @default.
- W2275396013 hasRelatedWork W2741432740 @default.
- W2275396013 hasVolume "260" @default.
- W2275396013 isParatext "false" @default.
- W2275396013 isRetracted "false" @default.
- W2275396013 magId "2275396013" @default.
- W2275396013 workType "article" @default.