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- W2275397239 abstract "The application of N-methyl-N'-nitro-N-nitrozoguanidin (NG), as chemical mutagen along with the use of mutations of resistance to an antibiotic streptomycin in the selection of a producer of the bacteryolytic enzyme complex of Streptomyces albus 2435 with the increased biosynthetic ability are investigated. The conditions of NG mutagen treatment (1 mg/ml, 20 min.), allowing to obtain mutants with supersynthesis of a target product are determined.The nitrozoguanidin-induced strains of Streptomycesalbus105 and 107, steadyto streptomycin (in concentration of 100-250 mkg/ml) with the increased (1,6 times) abilityto synthesis of bacteryolysins are received. The efficiency of the offered combination of selective factors (nitrozoguanidin and mutation of streptomycin-resistance) for the selection of producers in the course of the supporting selection and for industrial application is shown.The need of a producer’s supporting selection is caused by heterogeneity of cultures of streptomycetes and decrease in biosynthetic activity in the course of storage. For the development of industrial technology only the producer with supersynthesis of a product, in this case a producer of antimicrobial enzyme preparations, can be used." @default.
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- W2275397239 date "2015-04-02" @default.
- W2275397239 modified "2023-09-26" @default.
- W2275397239 title "Obtaining of highly active streptomycin-resistant bacteriolysins’s producers of Streptomyces albus" @default.
- W2275397239 doi "https://doi.org/10.15587/2312-8372.2015.40552" @default.
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