Matches in SemOpenAlex for { <https://semopenalex.org/work/W2275796694> ?p ?o ?g. }
- W2275796694 endingPage "430" @default.
- W2275796694 startingPage "423" @default.
- W2275796694 abstract "Lactophoricin (LPcin), a component of proteose peptone (113–135) isolated from bovine milk, is a cationic amphipathic antimicrobial peptide consisting of 23 amino acids. We designed a series of N- or C-terminal truncated variants, mutated analogs, and truncated mutated analogs using peptide-engineering techniques. Then, we selected three LPcin analogs of LPcin-C8 (LPcin-YK1), LPcin-T2WT6W (LPcin-YK2), and LPcin-T2WT6W-C8 (LPcin-YK3), which may have better antimicrobial activities than LPcin, and successfully expressed them in E. coli with high yield. We elucidated the 3D structures and topologies of the three LPcin analogs in membrane environments by conducting NMR structural studies. We investigated the purity of the LPcin analogs and the α-helical secondary structures by performing 1H-15N 2D HSQC and HMQC-NOESY liquid-state NMR spectroscopy using protein-containing micelle samples. We measured the 3D structures and tilt angles in membranes by conducting 15N 1D and 2D 1H-15N SAMMY type solid-state NMR spectroscopy with an 800 MHz in-house-built 1H-15N double-resonance solid-state NMR probe with a strip-shield coil, using protein-containing large bicelle samples aligned and confirmed by molecular-dynamics simulations. The three LPcin analogs were found to be curved α-helical structures, with tilt angles of 55–75° for normal membrane bilayers, and their enhanced activities may be correlated with these topologies." @default.
- W2275796694 created "2016-06-24" @default.
- W2275796694 creator A5002165527 @default.
- W2275796694 creator A5024380828 @default.
- W2275796694 creator A5048197386 @default.
- W2275796694 creator A5060767977 @default.
- W2275796694 date "2016-01-01" @default.
- W2275796694 modified "2023-09-26" @default.
- W2275796694 title "NMR Structural Studies of Antimicrobial Peptides: LPcin Analogs" @default.
- W2275796694 cites W1558879154 @default.
- W2275796694 cites W1605952717 @default.
- W2275796694 cites W1857017278 @default.
- W2275796694 cites W1862291362 @default.
- W2275796694 cites W1965378813 @default.
- W2275796694 cites W1965921836 @default.
- W2275796694 cites W1967737979 @default.
- W2275796694 cites W1970141146 @default.
- W2275796694 cites W1970351230 @default.
- W2275796694 cites W1972672790 @default.
- W2275796694 cites W1986669067 @default.
- W2275796694 cites W1990196991 @default.
- W2275796694 cites W1991081822 @default.
- W2275796694 cites W1998364625 @default.
- W2275796694 cites W1999759849 @default.
- W2275796694 cites W2000324842 @default.
- W2275796694 cites W2008708240 @default.
- W2275796694 cites W2011516085 @default.
- W2275796694 cites W2012269679 @default.
- W2275796694 cites W2014322154 @default.
- W2275796694 cites W2014338329 @default.
- W2275796694 cites W2020755269 @default.
- W2275796694 cites W2025572806 @default.
- W2275796694 cites W2030963195 @default.
- W2275796694 cites W2033471463 @default.
- W2275796694 cites W2045187883 @default.
- W2275796694 cites W2047262266 @default.
- W2275796694 cites W2051363571 @default.
- W2275796694 cites W2054191418 @default.
- W2275796694 cites W2054402905 @default.
- W2275796694 cites W2059625238 @default.
- W2275796694 cites W2059992380 @default.
- W2275796694 cites W2061073126 @default.
- W2275796694 cites W2063265586 @default.
- W2275796694 cites W2063272306 @default.
- W2275796694 cites W2067015677 @default.
- W2275796694 cites W2073486807 @default.
- W2275796694 cites W2074220745 @default.
- W2275796694 cites W2074333690 @default.
- W2275796694 cites W2079031526 @default.
- W2275796694 cites W2087849089 @default.
- W2275796694 cites W2088248289 @default.
- W2275796694 cites W2092997319 @default.
- W2275796694 cites W2094588760 @default.
- W2275796694 cites W2095512917 @default.
- W2275796694 cites W2101327053 @default.
- W2275796694 cites W2101871915 @default.
- W2275796694 cites W2107317356 @default.
- W2275796694 cites W2111960068 @default.
- W2275796694 cites W2115042382 @default.
- W2275796694 cites W2128768386 @default.
- W2275796694 cites W2138825634 @default.
- W2275796694 cites W2139619765 @default.
- W2275796694 cites W2141640808 @default.
- W2275796694 cites W2143453595 @default.
- W2275796694 cites W2145736371 @default.
- W2275796694 cites W2159114734 @default.
- W2275796694 cites W2169821755 @default.
- W2275796694 cites W2170259317 @default.
- W2275796694 cites W2320034360 @default.
- W2275796694 doi "https://doi.org/10.1016/j.bpj.2015.12.006" @default.
- W2275796694 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4724650" @default.
- W2275796694 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26789765" @default.
- W2275796694 hasPublicationYear "2016" @default.
- W2275796694 type Work @default.
- W2275796694 sameAs 2275796694 @default.
- W2275796694 citedByCount "11" @default.
- W2275796694 countsByYear W22757966942016 @default.
- W2275796694 countsByYear W22757966942017 @default.
- W2275796694 countsByYear W22757966942018 @default.
- W2275796694 countsByYear W22757966942019 @default.
- W2275796694 countsByYear W22757966942020 @default.
- W2275796694 countsByYear W22757966942021 @default.
- W2275796694 crossrefType "journal-article" @default.
- W2275796694 hasAuthorship W2275796694A5002165527 @default.
- W2275796694 hasAuthorship W2275796694A5024380828 @default.
- W2275796694 hasAuthorship W2275796694A5048197386 @default.
- W2275796694 hasAuthorship W2275796694A5060767977 @default.
- W2275796694 hasBestOaLocation W22757966941 @default.
- W2275796694 hasConcept C11268172 @default.
- W2275796694 hasConcept C121332964 @default.
- W2275796694 hasConcept C133571119 @default.
- W2275796694 hasConcept C15920480 @default.
- W2275796694 hasConcept C178790620 @default.
- W2275796694 hasConcept C183882617 @default.
- W2275796694 hasConcept C184651966 @default.
- W2275796694 hasConcept C185592680 @default.
- W2275796694 hasConcept C193125475 @default.
- W2275796694 hasConcept C19549132 @default.