Matches in SemOpenAlex for { <https://semopenalex.org/work/W2275798200> ?p ?o ?g. }
- W2275798200 endingPage "223" @default.
- W2275798200 startingPage "212" @default.
- W2275798200 abstract "Abstract During degeneration process, the catabolism of collagen type II and anabolism of collagen type I in nucleus pulposus (NP) may influence the bioactivity of transplanted cells. Human adipose‐derived mesenchymal stem cells (hADMSCs) were cultured as a micromass or in a series of gradual proportion hydrogels of a mix of collagen types I and II. Cell proliferation and cytotoxicity were detected using CCK‐8 and LDH assays respectively. The expression of differentiation‐related genes and proteins, including SOX9, aggrecan, collagen type I, and collagen type II, was examined using RT‐qPCR and Western blotting. Novel phenotypic genes were also detected by RT‐qPCR and western blotting. Alcian blue and dimethylmethylene blue assays were used to investigate sulfate proteoglycan expression, and PI3K/AKT, MAPK/ERK, and Smad signaling pathways were examined by Western blotting. The results showed collagen hydrogels have good biocompatibility, and cell proliferation increased after collagen type II treatment. Expressions of SOX9, aggrecan, and collagen type II were increased in a collagen type II dependent manner. Sulfate proteoglycan synthesis increased in proportion to collagen type II concentration. Only hADMSCs highly expressed NP cell marker KRT19 in collagen type II culture. Additionally, phosphorylated Smad3, which is associated with phosphorylated ERK, was increased after collagen type II‐stimulation. The concentration and type of collagen affect hADMSC differentiation into NP cells. Collagen type II significantly ameliorates hADMSC differentiation into NP cells and promotes extracellular matrix synthesis. Therefore, anabolism of collagen type I and catabolism of type II may attenuate the differentiation and biosynthesis of transplanted stem cells. © 2016 BioFactors, 42(2):212–223, 2016" @default.
- W2275798200 created "2016-06-24" @default.
- W2275798200 creator A5000097895 @default.
- W2275798200 creator A5004318775 @default.
- W2275798200 creator A5012946544 @default.
- W2275798200 creator A5042804899 @default.
- W2275798200 creator A5054263957 @default.
- W2275798200 creator A5058353865 @default.
- W2275798200 creator A5074950896 @default.
- W2275798200 date "2016-02-16" @default.
- W2275798200 modified "2023-10-16" @default.
- W2275798200 title "Proportion of collagen type II in the extracellular matrix promotes the differentiation of human adipose‐derived mesenchymal stem cells into nucleus pulposus cells" @default.
- W2275798200 cites W125113021 @default.
- W2275798200 cites W1269576713 @default.
- W2275798200 cites W1569205135 @default.
- W2275798200 cites W1856130664 @default.
- W2275798200 cites W1958303027 @default.
- W2275798200 cites W1969154370 @default.
- W2275798200 cites W1973996314 @default.
- W2275798200 cites W1977921602 @default.
- W2275798200 cites W1982500566 @default.
- W2275798200 cites W1983915845 @default.
- W2275798200 cites W1984630791 @default.
- W2275798200 cites W1984642820 @default.
- W2275798200 cites W1991332628 @default.
- W2275798200 cites W1995069685 @default.
- W2275798200 cites W1996752433 @default.
- W2275798200 cites W1999927824 @default.
- W2275798200 cites W2003818377 @default.
- W2275798200 cites W2004152316 @default.
- W2275798200 cites W2013318198 @default.
- W2275798200 cites W2022055461 @default.
- W2275798200 cites W2024805424 @default.
- W2275798200 cites W2027478418 @default.
- W2275798200 cites W2030493675 @default.
- W2275798200 cites W2031751222 @default.
- W2275798200 cites W2032221859 @default.
- W2275798200 cites W2032255522 @default.
- W2275798200 cites W2037178326 @default.
- W2275798200 cites W2038490482 @default.
- W2275798200 cites W2045827234 @default.
- W2275798200 cites W2047020892 @default.
- W2275798200 cites W2052369639 @default.
- W2275798200 cites W2055011209 @default.
- W2275798200 cites W2056122485 @default.
- W2275798200 cites W2067909000 @default.
- W2275798200 cites W2070818796 @default.
- W2275798200 cites W2075593123 @default.
- W2275798200 cites W2081928132 @default.
- W2275798200 cites W2084444237 @default.
- W2275798200 cites W2086605700 @default.
- W2275798200 cites W2090252769 @default.
- W2275798200 cites W2104962158 @default.
- W2275798200 cites W2107277218 @default.
- W2275798200 cites W2108726565 @default.
- W2275798200 cites W2111608210 @default.
- W2275798200 cites W2125208830 @default.
- W2275798200 cites W2129750588 @default.
- W2275798200 cites W2138977969 @default.
- W2275798200 cites W2140476594 @default.
- W2275798200 cites W2158128644 @default.
- W2275798200 cites W2272226381 @default.
- W2275798200 cites W2315471692 @default.
- W2275798200 cites W2325557282 @default.
- W2275798200 cites W2329677750 @default.
- W2275798200 doi "https://doi.org/10.1002/biof.1266" @default.
- W2275798200 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26879681" @default.
- W2275798200 hasPublicationYear "2016" @default.
- W2275798200 type Work @default.
- W2275798200 sameAs 2275798200 @default.
- W2275798200 citedByCount "20" @default.
- W2275798200 countsByYear W22757982002016 @default.
- W2275798200 countsByYear W22757982002017 @default.
- W2275798200 countsByYear W22757982002018 @default.
- W2275798200 countsByYear W22757982002019 @default.
- W2275798200 countsByYear W22757982002020 @default.
- W2275798200 countsByYear W22757982002021 @default.
- W2275798200 countsByYear W22757982002022 @default.
- W2275798200 countsByYear W22757982002023 @default.
- W2275798200 crossrefType "journal-article" @default.
- W2275798200 hasAuthorship W2275798200A5000097895 @default.
- W2275798200 hasAuthorship W2275798200A5004318775 @default.
- W2275798200 hasAuthorship W2275798200A5012946544 @default.
- W2275798200 hasAuthorship W2275798200A5042804899 @default.
- W2275798200 hasAuthorship W2275798200A5054263957 @default.
- W2275798200 hasAuthorship W2275798200A5058353865 @default.
- W2275798200 hasAuthorship W2275798200A5074950896 @default.
- W2275798200 hasConcept C104317684 @default.
- W2275798200 hasConcept C105702510 @default.
- W2275798200 hasConcept C119524353 @default.
- W2275798200 hasConcept C134018914 @default.
- W2275798200 hasConcept C142724271 @default.
- W2275798200 hasConcept C148738053 @default.
- W2275798200 hasConcept C1491633281 @default.
- W2275798200 hasConcept C153911025 @default.
- W2275798200 hasConcept C171089720 @default.
- W2275798200 hasConcept C185592680 @default.
- W2275798200 hasConcept C189165786 @default.