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• W2276096011 abstract "Qualitative and quantitative aspects of actin gene expression in the sea star Pisaster ochraceus were investigated. Total testes RNA was isolated monthly from individual sea stars through two spermatogenic cycles and subjected to Northern blot analysis using an actin coding sequence probe and two actin gene-specific probes previously characterized in g. ochraceus. Three transcript size classes were identified. The largest (3.5 kb) was found to hybridize only with the actin coding probe and only at moderate stringencies. A 2.3 kb transcript encoding the cytoplasmic ( ~ y ) gene class was confirmed by hybridization to a gene specific probe and cDNA sequence comparisons with genomic sequence. The 2.1 kb size class hybridizes with the muscle (M) gene-specific probe. However, testes cDNA isolates representing actin coding sequence differ from those of an existing genomic isolate, suggesting expression of the other gene in this class. The Cy transcript is the dominant actin transcript, reaching its highest levels early in the cycle. Both the 3.5 kb transcript and M transcript(s1 appear at the onset of spermatogenesis. The M transcript maintains low and stable levels throughout the cycle while the 3.5 kb transcript levels increase as the cycle progresses. These patterns sharply contrast those observed for the transcripts of two other important sperm components, beta tubulin and histone H3." @default.
• W2276096011 created "2016-06-24" @default.
• W2276096011 creator A5027815150 @default.
• W2276096011 date "1988-01-01" @default.
• W2276096011 modified "2023-10-10" @default.
• W2276096011 title "Actin gene transcription during spermatogenesis in the sea star pisaster ochraceus" @default.
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• W2276096011 hasPublicationYear "1988" @default.
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