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• W2276343756 abstract "Follicle-stimulating hormone (FSH) is a glycoprotein hormone with two subunits, alpha and beta, and is required for gamete development. FSH is now known to exist as a ratio of two glycoforms that varies during the menstrual cycle and in premenopausal periods in women. A newly identified glycoform, referred to as diglycosylated FSH, is characterized by the presence of two glycans attached to the alpha subunit while none are attached to the beta subunit. Preliminary data suggest that estrogen is responsible for reducing the glycosylation of FSHbeta in reproductive-age women, thus yielding higher concentrations of diglycosylated FSH than is found in older women. This selective reduction of glycosylation of the FSHbeta subunit is suspected to be due to activity of different oligosaccharyltransferase (OST) subunit isoforms. To investigate how the OST subunit isoforms STT3A and STT3B subunit isoforms affect FSH glycosylation, we used a stably transfected GH3 cell line that produces recombinant hFSH, and selectively knocked down each isoform via siRNA transfection, and examined the glycosylation patterns of the hFSH produced. We used a liposomal transfection methodology for siRNA transfection, and chromatographic methods for FSH purification. Following transfection, RNA from transfected cells was isolated and evaluated using reverse transcription and quantitative real-time PCR (RT-qPCR) to measure expression levels of the two isoforms and glycosylation patterns of FSH subunits were examined via Western blot. Ultimately, use of siRNA to selectively knock-down STT3 isoforms did not produce observable variance in FSH subunit glycosylation patterns when compared to controls. Although the RT-qPCR results suggest that isoform knock-down was achieved on some level, the subunit glycoforms captured on the Western blots did not show significant variation in glycoform abundance." @default.
• W2276343756 created "2016-06-24" @default.
• W2276343756 creator A5048394912 @default.
• W2276343756 date "2013-12-01" @default.
• W2276343756 modified "2023-09-27" @default.
• W2276343756 title "Analysis of oligosaccharyltransferase isoform function in human follicle stimulating hormone via siRNA knockdown" @default.
• W2276343756 cites W1495780351 @default.
• W2276343756 cites W1554349566 @default.
• W2276343756 cites W1671655961 @default.
• W2276343756 cites W1922584555 @default.
• W2276343756 cites W1964404659 @default.
• W2276343756 cites W1965853292 @default.
• W2276343756 cites W1966543080 @default.
• W2276343756 cites W1967830055 @default.
• W2276343756 cites W1974769389 @default.
• W2276343756 cites W1976947803 @default.
• W2276343756 cites W1978653855 @default.
• W2276343756 cites W1978870437 @default.
• W2276343756 cites W1979260907 @default.
• W2276343756 cites W1979428788 @default.
• W2276343756 cites W1980906223 @default.
• W2276343756 cites W1981942998 @default.
• W2276343756 cites W1983569629 @default.
• W2276343756 cites W1986350281 @default.
• W2276343756 cites W1986446401 @default.
• W2276343756 cites W1987804131 @default.
• W2276343756 cites W1987816139 @default.
• W2276343756 cites W1987940160 @default.
• W2276343756 cites W1988660122 @default.
• W2276343756 cites W1990280547 @default.
• W2276343756 cites W1998024601 @default.
• W2276343756 cites W2005036107 @default.
• W2276343756 cites W2005446108 @default.
• W2276343756 cites W2005678694 @default.
• W2276343756 cites W2008632715 @default.
• W2276343756 cites W2008971085 @default.
• W2276343756 cites W2015548961 @default.
• W2276343756 cites W2017642141 @default.
• W2276343756 cites W2020979406 @default.
• W2276343756 cites W2026726238 @default.
• W2276343756 cites W2027894892 @default.
• W2276343756 cites W2033053828 @default.
• W2276343756 cites W2033350189 @default.
• W2276343756 cites W2039376139 @default.
• W2276343756 cites W2040470885 @default.
• W2276343756 cites W2041970316 @default.
• W2276343756 cites W2043693398 @default.
• W2276343756 cites W2048662852 @default.
• W2276343756 cites W2050525925 @default.
• W2276343756 cites W2050825501 @default.
• W2276343756 cites W2054682534 @default.
• W2276343756 cites W2055217000 @default.
• W2276343756 cites W2058657335 @default.
• W2276343756 cites W2063097466 @default.
• W2276343756 cites W2063896926 @default.
• W2276343756 cites W2064376735 @default.
• W2276343756 cites W2065566655 @default.
• W2276343756 cites W2066839011 @default.
• W2276343756 cites W2068994547 @default.
• W2276343756 cites W2070925013 @default.
• W2276343756 cites W2070926743 @default.
• W2276343756 cites W2075554307 @default.
• W2276343756 cites W2077412978 @default.
• W2276343756 cites W2077417665 @default.
• W2276343756 cites W2077847564 @default.
• W2276343756 cites W2078292875 @default.
• W2276343756 cites W2078735767 @default.
• W2276343756 cites W2087317301 @default.
• W2276343756 cites W2088704436 @default.
• W2276343756 cites W2090690774 @default.
• W2276343756 cites W2091439201 @default.
• W2276343756 cites W2092224562 @default.
• W2276343756 cites W2092321734 @default.
• W2276343756 cites W2094010977 @default.
• W2276343756 cites W2094049074 @default.
• W2276343756 cites W2094874507 @default.
• W2276343756 cites W2095157299 @default.
• W2276343756 cites W2096634782 @default.
• W2276343756 cites W2102825248 @default.
• W2276343756 cites W2103284237 @default.
• W2276343756 cites W2103785922 @default.
• W2276343756 cites W2106203560 @default.
• W2276343756 cites W2106314418 @default.
• W2276343756 cites W2109184486 @default.
• W2276343756 cites W2114792768 @default.
• W2276343756 cites W2115294629 @default.
• W2276343756 cites W2116114174 @default.
• W2276343756 cites W2119416730 @default.
• W2276343756 cites W2119842592 @default.
• W2276343756 cites W2122250093 @default.
• W2276343756 cites W2123467533 @default.
• W2276343756 cites W2123825989 @default.
• W2276343756 cites W2124106344 @default.
• W2276343756 cites W2127324121 @default.
• W2276343756 cites W2127626240 @default.
• W2276343756 cites W2133344686 @default.
• W2276343756 cites W2134616533 @default.
• W2276343756 cites W2135644512 @default.
• W2276343756 cites W2137344987 @default.
• W2276343756 cites W2138081057 @default.

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