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- W2276384342 abstract "Cell division in Bacillus subtilis is a highly regulated process. Division takes place precisely at midcell resulting in two equally sized daughter cells. It is important that the divisome is disassembled after division is completed and does not directly re-assemble. Otherwise a new cycle of division is initiated close to the new formed cell pole, resulting in non viable, DNA-less mini cells. This study analyses the role of the intramembrane protease RasP in preventing divisome re-assembly. RasP degrades the late cell division protein FtsL in vivo. We tried to establish an in vitro assay to investigate this proteolysis. Both proteins were purified, but RasP seems to be partly unfolded after solubilisation. Therefore a heterolous co-expression system in E. coli was established instead. It is shown here that the division protein DivIC can protect FtsL against RasP cleavage. This stabilisation is achieved by inhibiting substrate recognition. It could be shown that a recognition motif within the cytosolic N-terminal domain of FtsL is essential for degradation by RasP. FtsL and DivIC tightly interact with each other. Direct interaction of the N-terminal domains blocks accessibility of the FtsL substrate recognition motif. Hence, as long as FtsL is incorporated in the divisome, RasP cleavage is impaired. After the division complex disassembles RasP is able to degrade FtsL. This cleavage removes FtsL from the membrane. Using fluorescence microscopy it was shown that the cytosolic cleavage product is then rapidly degraded by general proteolysis. A complex network of the late division proteins FtsL, DivIC and DivIB most likely provides a scaffold for cytokinesis. Since these proteins are strongly interdependent on each other for correct assembly, complete degradation of FtsL should efficiently prevent re-assembly of the divisome close to the new cell pole." @default.
- W2276384342 created "2016-06-24" @default.
- W2276384342 creator A5032578414 @default.
- W2276384342 date "2010-01-01" @default.
- W2276384342 modified "2023-09-27" @default.
- W2276384342 title "The cell division protein FtsL of Bacillus subtilis and its proteolysis" @default.
- W2276384342 hasPublicationYear "2010" @default.
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