Matches in SemOpenAlex for { <https://semopenalex.org/work/W2276403203> ?p ?o ?g. }
Showing items 1 to 45 of
45
with 100 items per page.
- W2276403203 abstract "Advanced glycosylation end products (AGEs) have been implicated in the structural and functional alterations of proteins that occur during aging and long-term diabetes. In the present study, murine RAW 264.7 macrophages were incubated with poly-L-lysine (PLL)-AGEs to examine cyclooxygenase-2 (COX-2) protein expression. Treatment of RAW 264.7 cells with PLL-AGEs caused a dose-dependent increase in COX activity as reflected by PGE2 secretion (measured in the presence of exogenous arachidonic acid). Furthermore, treatment of RAW 264.7 cells with PLL-AGEs induced COX-2 but not COX-1 expression. The induction was affected by neither L-buthionine-[S, R]-sulfoximine (BSO), a gamma-glutamylcysteine synthetase inhibitor, nor by L-nitro-acetyl-cysteine (L-NAC), a known glutathione precursor, suggesting that AGEs-induced COX-2 expression is not due to reactive oxygen species. Moreover, COX-2 expression was affected by neither N-gamma-nitro-L-arginine methyl ester (L-NAME), a competitive inhibitor of nitric oxide synthase (NOS), nor polymyxin B, a lipopolysaccharide (LPS) inhibitor, suggesting that COX-2 induction is not secondary to iNOS induction or LPS contamination. The tyrosine kinase inhibitor, genistein and tyrphostin AG 126, and the p38 mitogen-activated protein kinase (MAPK) inhibitor, SB 203580, inhibited PLL-AGEs-induced COX-2 expression, while the Ras inhibitor, FPT inhibitor II, and the MEK inhibitor, PD 98059, had no effect on PLL-AGEs-induced COX-2 expression. Incubation of RAW 264.7 cells with PLL-AGEs resulted in activation of p38 MAPK, and this activation was suppressed by genistein and SB 203580. Taken together, our results suggest that activation of protein tyrosine kinase and p38 MAPK is involved in AGEs-induced COX-2 expression in RAW 264.7 macrophages." @default.
- W2276403203 created "2016-06-24" @default.
- W2276403203 creator A5076959569 @default.
- W2276403203 date "2001-01-01" @default.
- W2276403203 modified "2023-09-23" @default.
- W2276403203 title "p38 Mitogen-Activated Protein Kinase在過度醣化最終產物誘導RAW 264.7巨噬細胞表現環氧酵素-2所扮演的角色" @default.
- W2276403203 hasPublicationYear "2001" @default.
- W2276403203 type Work @default.
- W2276403203 sameAs 2276403203 @default.
- W2276403203 citedByCount "0" @default.
- W2276403203 crossrefType "journal-article" @default.
- W2276403203 hasAuthorship W2276403203A5076959569 @default.
- W2276403203 hasConcept C153911025 @default.
- W2276403203 hasConcept C181199279 @default.
- W2276403203 hasConcept C184235292 @default.
- W2276403203 hasConcept C185592680 @default.
- W2276403203 hasConcept C2777476445 @default.
- W2276403203 hasConcept C2777622882 @default.
- W2276403203 hasConcept C2781249067 @default.
- W2276403203 hasConcept C51551487 @default.
- W2276403203 hasConcept C538909803 @default.
- W2276403203 hasConcept C55493867 @default.
- W2276403203 hasConcept C57074206 @default.
- W2276403203 hasConcept C86803240 @default.
- W2276403203 hasConcept C97029542 @default.
- W2276403203 hasConceptScore W2276403203C153911025 @default.
- W2276403203 hasConceptScore W2276403203C181199279 @default.
- W2276403203 hasConceptScore W2276403203C184235292 @default.
- W2276403203 hasConceptScore W2276403203C185592680 @default.
- W2276403203 hasConceptScore W2276403203C2777476445 @default.
- W2276403203 hasConceptScore W2276403203C2777622882 @default.
- W2276403203 hasConceptScore W2276403203C2781249067 @default.
- W2276403203 hasConceptScore W2276403203C51551487 @default.
- W2276403203 hasConceptScore W2276403203C538909803 @default.
- W2276403203 hasConceptScore W2276403203C55493867 @default.
- W2276403203 hasConceptScore W2276403203C57074206 @default.
- W2276403203 hasConceptScore W2276403203C86803240 @default.
- W2276403203 hasConceptScore W2276403203C97029542 @default.
- W2276403203 hasLocation W22764032031 @default.
- W2276403203 hasOpenAccess W2276403203 @default.
- W2276403203 hasPrimaryLocation W22764032031 @default.
- W2276403203 isParatext "false" @default.
- W2276403203 isRetracted "false" @default.
- W2276403203 magId "2276403203" @default.
- W2276403203 workType "article" @default.