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- W2276764835 abstract "Here, we describe a protocol for the reliable measurement of the amount of Ca(2+) in the sarcoplasmic reticulum (SR) Ca(2+) store of cardiac myocytes. The whole-cell patch-clamp method is used to provide controlled loading of the SR during conditioning depolarizing pulses, followed by rapid application of a high dose of caffeine to release all SR Ca(2+) and to prevent Ca(2+) reuptake by the SR. Simultaneous measurement of membrane currents records Ca(2+) extruded through the Na(+)-Ca(2+) exchanger. The integral of the caffeine-induced Na(+)-Ca(2+) exchange current is then used as a measure of the SR Ca(2+). Derived measurements include the Ca(2+) buffering capacity and measurement of fractional release as an indicator of coupling gain. Caveats, advantages, and disadvantages of this method and alternative methods are discussed." @default.
- W2276764835 created "2016-06-24" @default.
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- W2276764835 date "2015-04-01" @default.
- W2276764835 modified "2023-09-23" @default.
- W2276764835 title "Measuring Sarcoplasmic Reticulum Ca2+Content, Fractional Release, and Ca2+Buffering in Cardiac Myocytes" @default.
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- W2276764835 doi "https://doi.org/10.1101/pdb.prot076976" @default.
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