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- W2276780512 abstract "Rickettsial diseases are being under reported from India but they are significant contributors of pyrexia. The present study deals with utilization of Weil Felix test (WF) as screening test for diagnosis of rickettsial diseases in patients of fever and rash. Latex agglutination test PROGEN from Tulip Diagnostics (P) Ltd. was used for screening 156 samples out of which 27 samples (17.30%) were positive. Maximum positivity was shown towards OX-2 antigen (88.88%) followed by OX-19 (66.66%) and least towards OX-K (11.11%). Weil Felix test though being non specific is user friendly, non-tedious and cannot be discarded altogether from laboratory set-up. Most fevers in acutely ill patients with rash often present a diagnostic challenge for physicians. Rickettsial infections constitute an important etiology when fever presents in concert with distinctive appearance of an eruption. In general terms laboratory confirmation of rickettsial infection maybe obtained by isolating and biotyping the rickettsia from the patient or by demonstrating an antibody response during the disease or in special circumstances by demonstrating and identifying the organisms in the lesions. The difficulties and dangers of working with live pathogenic rickettsias preclude isolation of the organism as a diagnostic measure in most clinical laboratories. (1) Hence cultivation of rickettsia in cell culture is technically feasible but seldom undertaken. For most clinical microbiology laboratories the diagnosis of suspected clinical cases of rickettsial infections will be restricted to testing of blood samples for antibody. Serologic assays for the diagnosis of rickettsial infections include the gold standard indirect immunofluorescence assay (IFA), indirect immunoperoxidase assay, latex agglutination, enzyme immunoassay (EIA), Proteus vulgaris OX-19 and OX-2, Proteus mirabilis OX-K strain agglutination, line blot, Western immunoblotting and rapid flow assays. Other serologic tests include indirect haemagglutination (IHA), microagglutination and complement fixation(CF). (2)" @default.
- W2276780512 created "2016-06-24" @default.
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- W2276780512 date "2015-07-20" @default.
- W2276780512 modified "2023-09-23" @default.
- W2276780512 title "Role of Weil Felix Test for Rickettsial Infections" @default.
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- W2276780512 doi "https://doi.org/10.6084/m9.figshare.1488604.v1" @default.
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