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- W2276945557 abstract "Ionic currents induced by cell swelling were characterized in primary cultures of rabbit distal bright convoluted tubule (DCTb) by the whole cell patch-clamp technique. Cl − currents were produced spontaneously by whole cell recording with an isotonic pipette solution or by exposure to a hypotonic stress. Initial Cl − currents exhibited outwardly rectifying current-voltage relationship, whereas steady-state currents showed strong decay with depolarizing pulses. The ion selectivity sequence was I − = Br − > Cl − ≫ glutamate. Currents were inhibited by 0.1 mM 5-nitro-2-(3-phenylpropylamino)benzoic acid and 1 mM 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid and strongly blocked by 1 mM diphenylamine-2-carboxylate. Currents were insensitive to intracellular Ca 2+ but required the presence of extracellular Ca 2+ . They were not activated in cells pretreated with 200 nM staurosporine, 50 μM LaCl 3 , 10 μM nifedipine, 100 μM verapamil, 5 μM tamoxifen, and 50 μM dideoxyforskolin. Staurosporine, tamoxifen, verapamil, or the absence of external Ca 2+ was without effect on the fully developed Cl − currents. Osmotic shock also activated K + currents in Cl − -free conditions. These currents were time independent, activated at depolarized potentials, and inhibited by 5 mM BaCl 2 . The activation of Cl − and K + currents by an osmotic shock may be implicated in regulatory volume decrease in DCTb cells." @default.
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- W2276945557 date "1997-11-01" @default.
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- W2276945557 title "Cl<sup>−</sup>and K<sup>+</sup>conductances activated by cell swelling in primary cultures of rabbit distal bright convoluted tubules" @default.
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- W2276945557 doi "https://doi.org/10.1152/ajprenal.1997.273.5.f680" @default.
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