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- W2277777727 abstract "Actinobacillus pleuropneumoniae (APP) causes porcine pleuropneumonia (PP).Serotypes and antimicrobial resistance patterns in APP isolates from pigs in Korea were examined.Sixty-five APP isolates were genetically serotyped using standard and multiplex PCR (polymerase chain reaction). Antimicrobial susceptibilities were tested using the standardized disk-agar method. PCR was used to detect β-lactam, gentamicin and tetracycline-resistance genes. The random amplified polymorphic DNA (RAPD) patterns were determined by PCR.Korean pigs predominantly carried APP serotypes 1 and 5. Among 65 isolates, one isolate was sensitive to all 12 antimicrobials tested in this study. Sixty-two isolates was resistant to tetracycline and 53 isolates carried one or five genes including tet(B), tet(A), tet(H), tet(M)/tet(O), tet(C), tet(G) and/or tet(L)-1 markers. Among 64 strains, 9% and 26.6% were resistance to 10 and three or more antimicrobials, respectively. Thirteen different antimicrobial resistance patterns were observed and RAPD analysis revealed a separation of the isolates into two clusters: cluster II (6 strains resistant to 10 antimicrobials) and cluster I (the other 59 strains).Results show that APP serotypes 1 and 5 are the most common in Korea, and multi-drug resistant strains are prevalent. RAPD analysis demonstrated that six isolates resistant to 10 antimicrobials belonged to the same cluster." @default.
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- W2277777727 date "2016-03-04" @default.
- W2277777727 modified "2023-09-29" @default.
- W2277777727 title "Molecular serotyping and antimicrobial resistance profiles of<i>Actinobacillus pleuropneumoniae</i>isolated from pigs in South Korea" @default.
- W2277777727 cites W1576800571 @default.
- W2277777727 doi "https://doi.org/10.1080/01652176.2016.1155241" @default.
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