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- W2277785857 abstract "Objective: To construct a lentiviral vector expressing Netrin-1 gene for studying the role of Netrin-1 in the development of neuron. Methods: The gene fragment of netrin-1 was obtained by PCR and cloned into lentiviral vector pLXSN. Then the recombinant plasmid was confirmed by PCR,restriction endonuclease digestion and sequencing. The recombinant retroviral vector pLX-NT was selected to transfect packaging cell PA317 to gain virus particles with infection ability. Human glioblastoma multiform cells SW038-C2 were infected.The expression of netrin-1 in SW038-C2 cells was determined by Western blotting. Results: The recombinant plasmid,named pLX-NT,was constructed and identified.Packaged by packaging cell PA3 1 7,the recombinant vector could transfect human glioblastoma multiform cells SW038-C2 effectively and the expression of netrin-1 in SW038-C2 cells was determined by Western blotting. Conclusion: The plasmid pLX-NT has been constructed successfully." @default.
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- W2277785857 date "2010-01-01" @default.
- W2277785857 modified "2023-09-23" @default.
- W2277785857 title "Construction and identification of lentiviral vector with Netrin-1 gene." @default.
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