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- W2277854628 abstract "Received: Revised: Accepted: April 15, 2014 July 03, 2014 December 23, 2014 Clusterin (CLU) is a multifunctional protein whose expression has been related to many physiological and pathological processes. The most abundant gene product is the secreted form of CLU (sCLU) described as a prosurvival and cytoprotective protein. A nuclear CLU (nCLU) isoform involved in death and inhibition of cell growth has also been reported. The aims of the present work were to verify the differential presence of sCLU and nCLU in a primary culture of porcine aortic endothelial cells (pAEC) and to evaluate whether lipopolysaccharide (LPS) can influence their expression. LPS-treated pAEC expressed both mRNA isoforms but only sCLU protein was detected. LPS exposure induced transient mRNA and protein increases of sCLU at 1 h of treatment. In conclusion our data showed that in LPS-treated pAEC sCLU is the most abundant form of clusterin and nCLU can be considered irrelevant and is rarely translated. ©2014 PVJ. All rights reserved" @default.
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- W2277854628 date "2015-01-01" @default.
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- W2277854628 title "Expression of clusterin isoforms in LPS treated porcine aortic endothelial cells." @default.
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