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- W22781246 startingPage "581" @default.
- W22781246 abstract "The global superhelical state of intracellular DNA is stringently controlled by topoisomerase action. Little is know, however, about topoisomerase-directed relaxation of localized DNA supercoiling generated by protein tracking processes such as transcription. Here we use transcription by a yeast Gal4 and phage T7 RNA polymerase fusion protein to induce localized supercoiling which, in turn, triggers site-specific DNA recombination by gamma delta resolvase. We demonstrate that only large amounts of eukaryotic topoisomerase I interfere, through supercoiling relaxation, with the topological coupling between transcription and recombination. The additional presence of a strong cleavage site for topoisomerase I has little influence on the relaxation of localized supercoiling. We also show that high levels of human topoisomerase II fail to compete with transcription-driven recombination. However, drastically reduced amounts of either enzyme completely suppress recombination of overall supercoiled DNA. Together, our results reveal a marked difference in topoisomerase requirement to relax transcription-induced and global DNA supercoiling. We discuss possible reasons for this difference and conclude that localized supercoiling frequently may escape relaxation by eukaryotic topoisomerases to mediate topological couplings between DNA transactions." @default.
- W22781246 created "2016-06-24" @default.
- W22781246 creator A5016667793 @default.
- W22781246 creator A5079484352 @default.
- W22781246 date "1996-02-01" @default.
- W22781246 modified "2023-10-12" @default.
- W22781246 title "Differential control of transcription-induced and overall DNA supercoiling by eukaryotic topoisomerases in vitro." @default.
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- W22781246 doi "https://doi.org/10.1002/j.1460-2075.1996.tb00390.x" @default.
- W22781246 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/449976" @default.
- W22781246 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8599941" @default.
- W22781246 hasPublicationYear "1996" @default.
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