Matches in SemOpenAlex for { <https://semopenalex.org/work/W2278186554> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2278186554 endingPage "12" @default.
- W2278186554 startingPage "5" @default.
- W2278186554 abstract "Polymerase chain reaction (PCR) is a suitable method for detecting pathogenic agents and first step in this process is DNA extraction. DNA extraction enumerate as a long stage in molecular genetic researches that is longer than total PCR process in some cases. The aim of this research is use of direct PCR (without DNA extraction) for different typing of Clostridium perfringens. In this study direct PCR and also PCR by extracted DNA was implemented on the A, B, C and D types of Clostridium perfringens. For this purpose four pairs of specific primers that were complementary with parts of the genes encoding α, β, e and ι toxins of this bacterium were used. In both direct PCR and PCR by extracted DNA, 196, 324 and 655 bp fragments were amplified that are dependent to α, β and e toxins respectively. Upshot different strains of this bacterium were identified based on amplified fragment length in PCR. The result showed that success of PCR is simple from colony directly and quality of created bands is the same in both methods. Therefore, use of this method is recommended because the direct PCR reduce time, steps and cost of PCR and provide possibility of testing many samples in shorter times provides." @default.
- W2278186554 created "2016-06-24" @default.
- W2278186554 creator A5040724282 @default.
- W2278186554 creator A5044189931 @default.
- W2278186554 date "2013-01-01" @default.
- W2278186554 modified "2023-09-23" @default.
- W2278186554 title "COMPARE OF TWO METHODS OF DIRECT PCR AND PCR WITH DNA EXTRACTION IN CLOSTRIDIUM PERFRINGENS TYPING" @default.
- W2278186554 hasPublicationYear "2013" @default.
- W2278186554 type Work @default.
- W2278186554 sameAs 2278186554 @default.
- W2278186554 citedByCount "1" @default.
- W2278186554 countsByYear W22781865542016 @default.
- W2278186554 crossrefType "journal-article" @default.
- W2278186554 hasAuthorship W2278186554A5040724282 @default.
- W2278186554 hasAuthorship W2278186554A5044189931 @default.
- W2278186554 hasConcept C104317684 @default.
- W2278186554 hasConcept C153911025 @default.
- W2278186554 hasConcept C200658500 @default.
- W2278186554 hasConcept C2779116991 @default.
- W2278186554 hasConcept C2781209916 @default.
- W2278186554 hasConcept C28134486 @default.
- W2278186554 hasConcept C33935397 @default.
- W2278186554 hasConcept C48023723 @default.
- W2278186554 hasConcept C49105822 @default.
- W2278186554 hasConcept C52001828 @default.
- W2278186554 hasConcept C523546767 @default.
- W2278186554 hasConcept C54355233 @default.
- W2278186554 hasConcept C552990157 @default.
- W2278186554 hasConcept C78063203 @default.
- W2278186554 hasConcept C86803240 @default.
- W2278186554 hasConcept C89423630 @default.
- W2278186554 hasConcept C90583042 @default.
- W2278186554 hasConceptScore W2278186554C104317684 @default.
- W2278186554 hasConceptScore W2278186554C153911025 @default.
- W2278186554 hasConceptScore W2278186554C200658500 @default.
- W2278186554 hasConceptScore W2278186554C2779116991 @default.
- W2278186554 hasConceptScore W2278186554C2781209916 @default.
- W2278186554 hasConceptScore W2278186554C28134486 @default.
- W2278186554 hasConceptScore W2278186554C33935397 @default.
- W2278186554 hasConceptScore W2278186554C48023723 @default.
- W2278186554 hasConceptScore W2278186554C49105822 @default.
- W2278186554 hasConceptScore W2278186554C52001828 @default.
- W2278186554 hasConceptScore W2278186554C523546767 @default.
- W2278186554 hasConceptScore W2278186554C54355233 @default.
- W2278186554 hasConceptScore W2278186554C552990157 @default.
- W2278186554 hasConceptScore W2278186554C78063203 @default.
- W2278186554 hasConceptScore W2278186554C86803240 @default.
- W2278186554 hasConceptScore W2278186554C89423630 @default.
- W2278186554 hasConceptScore W2278186554C90583042 @default.
- W2278186554 hasIssue "437" @default.
- W2278186554 hasLocation W22781865541 @default.
- W2278186554 hasOpenAccess W2278186554 @default.
- W2278186554 hasPrimaryLocation W22781865541 @default.
- W2278186554 hasRelatedWork W1972421685 @default.
- W2278186554 hasRelatedWork W1987110868 @default.
- W2278186554 hasRelatedWork W2005589344 @default.
- W2278186554 hasRelatedWork W2042480249 @default.
- W2278186554 hasRelatedWork W2052199264 @default.
- W2278186554 hasRelatedWork W2096497004 @default.
- W2278186554 hasRelatedWork W2136001436 @default.
- W2278186554 hasRelatedWork W2161610375 @default.
- W2278186554 hasRelatedWork W2255128425 @default.
- W2278186554 hasRelatedWork W2352094479 @default.
- W2278186554 hasRelatedWork W2358214203 @default.
- W2278186554 hasRelatedWork W2359266561 @default.
- W2278186554 hasRelatedWork W2364108078 @default.
- W2278186554 hasRelatedWork W2389315847 @default.
- W2278186554 hasRelatedWork W2467212064 @default.
- W2278186554 hasRelatedWork W2791707599 @default.
- W2278186554 hasRelatedWork W2913553022 @default.
- W2278186554 hasRelatedWork W2984794609 @default.
- W2278186554 hasRelatedWork W2821026216 @default.
- W2278186554 hasRelatedWork W3032539425 @default.
- W2278186554 hasVolume "8" @default.
- W2278186554 isParatext "false" @default.
- W2278186554 isRetracted "false" @default.
- W2278186554 magId "2278186554" @default.
- W2278186554 workType "article" @default.