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• W2279054813 abstract "CBA mice are resistant to Leishmania major yet permissive to L. amazonensis infection. In addition, CBA macrophages control L. major, but not L. amazonensis infection in vitro. In a comparative study performed by our group increase in expression of the scavenger receptor MARCO has been detected in response to L. major, but not to L. amazonensis infection. Moreover, ED31 monoclonal antibody against MARCO reduced by 30% L. major infection in CBA macrophages. These findings indicate that MARCO plays a role in L. major recognition by CBA macrophages. We hypothesized that MARCO receptor participates in the recognition and phagocytosis of L. major by macrophages, directing the outcome of infection. In the present study, we aimed to further disclose the role MARCO plays in L. major infection of murine macrophages. First J774 cells were transfected with pcDNA3.1-MARCO vector (MARCO-J774) or pcDNA3.1 vector (MOCK-J774). Expression of MARCO gene shown to be five times higher in MARCO-J774 cells compared to MOCK-J774 cells. Overexpression of MARCO enhanced mitochondrial metabolic activity in 14% and 39% of both MARCO-J774 and MOCK-J774, respectively, when compared to the control non-transfected J774 cells. However, enhancement in mitochondrial metabolic activity did not alter the cell viability of transfected clones. MARCO overexpression did not increase the binding of L. major in J774 cells, but increased both the percentage of infection of L. major and the number of parasites / cell until 24 h after infection (p ≤ 0,05). Additionally, we investigated whether MARCO be inducing changes in cell membrane that would favor L. major uptake by macrophages. We observed that MARCO-J774 cells (67%) showed a pronounced spread of cell membranes, with short microvilli and lamellipodia, events observed in a reduced number of MOCK-J774 (24%). Then, we evaluated production of pro-inflammatory chemokine and cytokine induced by L. major in MARCO-J774 cells. L. major addition to MARCO-J774 cells induced higher significantly levels of MCP-1 and TNF-α compared to MOCK-J774 cells at 24 and 48 h post infection (p < 0,01). Higher levels of IL-6 were also observed at 48 h of L. major infection in MARCO-J774 cells compared to control (p < 0,05). Similarly, NO production induced by L. major was much more higher in MARCO-J774 cells compared to MOCK-J774 at 24 and 48 h post infection (p ≤ 0,01). Although in MARCO-overexpressing cells, enhancement in pro-inflammatory chemokines and cytokines in response to L. major has been observed, no effect on parasite intracellular survival has been detected. In summary, our findings suggest that the scavenger receptor MARCO overexpression favors the entry of L. major in J774 cells, and trigger an effector immune response that directs outcome of Leishmania infection." @default.
• W2279054813 created "2016-06-24" @default.
• W2279054813 creator A5029108126 @default.
• W2279054813 date "2014-01-01" @default.
• W2279054813 modified "2023-09-23" @default.
• W2279054813 title "Avaliação do papel do receptor marco na infecção de macrófagos murinos por Leishmania major." @default.
• W2279054813 cites W1258546148 @default.
• W2279054813 cites W1480188565 @default.
• W2279054813 cites W1485265843 @default.
• W2279054813 cites W1488111020 @default.
• W2279054813 cites W1494869000 @default.
• W2279054813 cites W1501109059 @default.
• W2279054813 cites W1508999422 @default.
• W2279054813 cites W1526545436 @default.
• W2279054813 cites W1530044612 @default.
• W2279054813 cites W1548326502 @default.
• W2279054813 cites W1550904409 @default.
• W2279054813 cites W1555734844 @default.
• W2279054813 cites W1564545512 @default.
• W2279054813 cites W1564900951 @default.
• W2279054813 cites W1566635410 @default.
• W2279054813 cites W1584602588 @default.
• W2279054813 cites W1586303953 @default.
• W2279054813 cites W1588364673 @default.
• W2279054813 cites W1676233161 @default.
• W2279054813 cites W1739247159 @default.
• W2279054813 cites W1741609474 @default.
• W2279054813 cites W1810297567 @default.
• W2279054813 cites W1883122097 @default.
• W2279054813 cites W1918312395 @default.
• W2279054813 cites W1930598094 @default.
• W2279054813 cites W1935267859 @default.
• W2279054813 cites W1942668068 @default.
• W2279054813 cites W1946039171 @default.
• W2279054813 cites W1958024510 @default.
• W2279054813 cites W1958092593 @default.
• W2279054813 cites W1964050061 @default.
• W2279054813 cites W1966388070 @default.
• W2279054813 cites W1967729440 @default.
• W2279054813 cites W1968134126 @default.
• W2279054813 cites W1968365186 @default.
• W2279054813 cites W1969406774 @default.
• W2279054813 cites W1975123482 @default.
• W2279054813 cites W1976085982 @default.
• W2279054813 cites W1980213997 @default.
• W2279054813 cites W1980301203 @default.
• W2279054813 cites W1981649463 @default.
• W2279054813 cites W1985779248 @default.
• W2279054813 cites W1986367263 @default.
• W2279054813 cites W1989219781 @default.
• W2279054813 cites W1991464867 @default.
• W2279054813 cites W1991484350 @default.
• W2279054813 cites W1991650764 @default.
• W2279054813 cites W1994276657 @default.
• W2279054813 cites W1995528124 @default.
• W2279054813 cites W2000920565 @default.
• W2279054813 cites W2001793378 @default.
• W2279054813 cites W2001848039 @default.
• W2279054813 cites W2002204067 @default.
• W2279054813 cites W2003948064 @default.
• W2279054813 cites W2004345134 @default.
• W2279054813 cites W2005060197 @default.
• W2279054813 cites W2006002292 @default.
• W2279054813 cites W2007331685 @default.
• W2279054813 cites W2010328277 @default.
• W2279054813 cites W2012423481 @default.
• W2279054813 cites W2014185803 @default.
• W2279054813 cites W2020134002 @default.
• W2279054813 cites W2020152576 @default.
• W2279054813 cites W2023659884 @default.
• W2279054813 cites W2025056717 @default.
• W2279054813 cites W2026689863 @default.
• W2279054813 cites W2027139443 @default.
• W2279054813 cites W2029856143 @default.
• W2279054813 cites W2030256169 @default.
• W2279054813 cites W2034649458 @default.
• W2279054813 cites W2034707358 @default.
• W2279054813 cites W2036922334 @default.
• W2279054813 cites W2042863201 @default.
• W2279054813 cites W2043264074 @default.
• W2279054813 cites W2043791410 @default.
• W2279054813 cites W2046954690 @default.
• W2279054813 cites W2048225641 @default.
• W2279054813 cites W2049361955 @default.
• W2279054813 cites W2050422207 @default.
• W2279054813 cites W2052071533 @default.
• W2279054813 cites W2057028848 @default.
• W2279054813 cites W2057618822 @default.
• W2279054813 cites W2058986676 @default.
• W2279054813 cites W2059740054 @default.
• W2279054813 cites W2060932116 @default.
• W2279054813 cites W2061961359 @default.
• W2279054813 cites W2061972338 @default.
• W2279054813 cites W206507329 @default.
• W2279054813 cites W2065968940 @default.
• W2279054813 cites W2066190914 @default.
• W2279054813 cites W2067736150 @default.
• W2279054813 cites W2068028241 @default.
• W2279054813 cites W2068199835 @default.
• W2279054813 cites W2070024570 @default.

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