FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2279390889> ?p ?o ?g. }

• W2279390889 endingPage "1249" @default.
• W2279390889 startingPage "1236" @default.
• W2279390889 abstract "Microorganisms use a host of enzymes, including processive glycoside hydrolases, to deconstruct recalcitrant polysaccharides to sugars. Processive glycoside hydrolases closely associate with polymer chains and repeatedly cleave glycosidic linkages without dissociating from the crystalline surface after each hydrolytic step; they are typically the most abundant enzymes in both natural secretomes and industrial cocktails by virtue of their significant hydrolytic potential. The ubiquity of aromatic residues lining the enzyme catalytic tunnels and clefts is a notable feature of processive glycoside hydrolases. We hypothesized that these aromatic residues have uniquely defined roles, such as substrate chain acquisition and binding in the catalytic tunnel, that are defined by their local environment and position relative to the substrate and the catalytic center. Here, we investigated this hypothesis with variants of Serratia marcescens family 18 processive chitinases ChiA and ChiB. We applied molecular simulation and free energy calculations to assess active site dynamics and ligand binding free energies. Isothermal titration calorimetry provided further insight into enthalpic and entropic contributions to ligand binding free energy. Thus, the roles of six aromatic residues, Trp-167, Trp-275, and Phe-396 in ChiA, and Trp-97, Trp-220, and Phe-190 in ChiB, have been examined. We observed that point mutation of the tryptophan residues to alanine results in unfavorable changes in the free energy of binding relative to wild-type. The most drastic effects were observed for residues positioned at the entrances of the deep substrate-binding clefts and known to be important for processivity. Interestingly, phenylalanine mutations in ChiA and ChiB had little to no effect on chito-oligomer binding, in accordance with the limited effects of their removal on chitinase functionality." @default.
• W2279390889 created "2016-06-24" @default.
• W2279390889 creator A5018268999 @default.
• W2279390889 creator A5052089482 @default.
• W2279390889 creator A5062073455 @default.
• W2279390889 creator A5064586896 @default.
• W2279390889 creator A5067524914 @default.
• W2279390889 creator A5083669397 @default.
• W2279390889 creator A5087283503 @default.
• W2279390889 creator A5089993099 @default.
• W2279390889 date "2016-02-15" @default.
• W2279390889 modified "2023-10-06" @default.
• W2279390889 title "Aromatic-Mediated Carbohydrate Recognition in Processive <i>Serratia marcescens</i> Chitinases" @default.
• W2279390889 cites W1875952581 @default.
• W2279390889 cites W1965640337 @default.
• W2279390889 cites W1969215536 @default.
• W2279390889 cites W1970973025 @default.
• W2279390889 cites W1972770152 @default.
• W2279390889 cites W1974515964 @default.
• W2279390889 cites W1976223991 @default.
• W2279390889 cites W1976351388 @default.
• W2279390889 cites W1976499671 @default.
• W2279390889 cites W1980618423 @default.
• W2279390889 cites W1981338975 @default.
• W2279390889 cites W1981803179 @default.
• W2279390889 cites W1982946286 @default.
• W2279390889 cites W1983564053 @default.
• W2279390889 cites W1984528579 @default.
• W2279390889 cites W1987250042 @default.
• W2279390889 cites W1988287340 @default.
• W2279390889 cites W1988479656 @default.
• W2279390889 cites W1989195611 @default.
• W2279390889 cites W1989639319 @default.
• W2279390889 cites W1992225794 @default.
• W2279390889 cites W1995388602 @default.
• W2279390889 cites W1998587780 @default.
• W2279390889 cites W2005291574 @default.
• W2279390889 cites W2005679019 @default.
• W2279390889 cites W2006155140 @default.
• W2279390889 cites W2006256864 @default.
• W2279390889 cites W2014936174 @default.
• W2279390889 cites W2018456240 @default.
• W2279390889 cites W2019565712 @default.
• W2279390889 cites W2020357919 @default.
• W2279390889 cites W2025225233 @default.
• W2279390889 cites W2026395206 @default.
• W2279390889 cites W2026995511 @default.
• W2279390889 cites W2027408247 @default.
• W2279390889 cites W2027761908 @default.
• W2279390889 cites W2029667189 @default.
• W2279390889 cites W2033071347 @default.
• W2279390889 cites W2035160545 @default.
• W2279390889 cites W2036620149 @default.
• W2279390889 cites W2039185557 @default.
• W2279390889 cites W2039995090 @default.
• W2279390889 cites W2042258871 @default.
• W2279390889 cites W2042444908 @default.
• W2279390889 cites W2043701535 @default.
• W2279390889 cites W2050333027 @default.
• W2279390889 cites W2051921179 @default.
• W2279390889 cites W2053453972 @default.
• W2279390889 cites W2053700373 @default.
• W2279390889 cites W2054378255 @default.
• W2279390889 cites W2055600356 @default.
• W2279390889 cites W2057539529 @default.
• W2279390889 cites W2071466250 @default.
• W2279390889 cites W2076488841 @default.
• W2279390889 cites W2080138929 @default.
• W2279390889 cites W2084828551 @default.
• W2279390889 cites W2093583318 @default.
• W2279390889 cites W2098303032 @default.
• W2279390889 cites W2098492026 @default.
• W2279390889 cites W2099740349 @default.
• W2279390889 cites W2102881084 @default.
• W2279390889 cites W2112185869 @default.
• W2279390889 cites W2119921534 @default.
• W2279390889 cites W2120641909 @default.
• W2279390889 cites W2123199112 @default.
• W2279390889 cites W2126098632 @default.
• W2279390889 cites W2126210768 @default.
• W2279390889 cites W2126834628 @default.
• W2279390889 cites W2132262459 @default.
• W2279390889 cites W2137366236 @default.
• W2279390889 cites W2141555360 @default.
• W2279390889 cites W2150981663 @default.
• W2279390889 cites W2151939702 @default.
• W2279390889 cites W2155082155 @default.
• W2279390889 cites W2157329356 @default.
• W2279390889 cites W2160857148 @default.
• W2279390889 cites W2164623799 @default.
• W2279390889 cites W2165246631 @default.
• W2279390889 cites W2165600016 @default.
• W2279390889 cites W2172100516 @default.
• W2279390889 cites W2284307953 @default.
• W2279390889 cites W2323290166 @default.
• W2279390889 cites W2323729166 @default.
• W2279390889 cites W2418361432 @default.
• W2279390889 cites W4211221678 @default.

• next