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- W2279510246 abstract "To ensure the authentication of fishery products lacking biological characters, rapid species identification methods are required. Two DNA- and protein-based methods, PCR-SSCP (polymerase chain reaction - single strand conformation polymorphism) of a 464 bp segment of the cytochrome b – gene and isoelectric focusing (IEF) of water-soluble proteins from fish fillets, were applied to identify fillets of (sub-) tropical fish species available on the European market. Among the samples analysed weretwo taxonomically identified species from the family Sciaenidae and one from Sphyraenidae. By comparison of DNA- and protein patterns of different samples, information about intra-species variability of patterns,and homogeneity of batches (e.g. fillet blocks or bags) can be obtained. PCR-SSCP and IEF may be useful for pre-checking of a large number of samples by food control laboratories.ZusammenfassungZur Sicherstellung der Authentizitat von Fischerei-Erzeugnissen ohne biologische Merkmale sind schnelle Verfahren zur Speziesidentifizierung hilfreich. Zwei Methoden der DNA- bzw. Protein-Analyse wurden eingesetzt, um Filets (sub-) tropischer Fischarten, die auf dem europaischen Markt angeboten werden, zu identifizieren. Bei diesen Methoden handelt es sich um die PCR-SSCP (Polymerase-Kettenreaktion – Einzelstrang-Konformationspolymorphismus) – Analyse der PCR-Produkte und die IEF (isoelektrische Fokussierung) der wasserloslichen Fischmuskelproteine. Unter den untersuchten Proben waren zwei taxonomisch bestimmte Arten aus der Familie Sciaenidae und eine Spezies aus der Familie Sphyraenidae. Durch Vergleich der DNA- bzw. Proteinmuster lassen sich Informationen uber die intra-spezifische Variabilitat solcher Muster und die Einheitlichkeit von Partien (beispielsweise Filetblocke oder Filetbeutel) gewinnen. PCR-SSCP und IEF konnenin Laboratorien der Lebensmitteluberwachung als Vortest gerade bei hohen Probenzahlen sinnvoll eingesetzt werden." @default.
- W2279510246 created "2016-06-24" @default.
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- W2279510246 date "2012-01-01" @default.
- W2279510246 modified "2023-09-25" @default.
- W2279510246 title "PCR-SSCP and Isoelectric Focusing as Screening Methods for Identification of (Sub-) Tropical Fish Species" @default.
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- W2279510246 doi "https://doi.org/10.3220/infn59_81-89_2012" @default.
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