FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2279528030> ?p ?o ?g. }

• W2279528030 abstract "A genetic transformation system via the pollen tube pathway was investigated. Plasmid pD0432 (Ow et al., 1986), containing the cauliflower mosaic virus 35S promoter fused to the firefly(Photinus pyralis Linnaeus) luciferase coding sequence and a nopaline synthase terminator sequence, was used as the vector. Plasmid DNA prepared with each of 4 different methods was applied to the surface of the remaining style of florets decapitated 18 or 24 hours following pollination. There were no significant differences in percentage of fruit set between exogenous DNA application time (18 or 24 hours following pollination), among treatments (plasmid DNA solution, control solution, and no solution applied), or interaction between application time and treatments. According to the observation of the movement of EB-labeled plasmid DNA, plasmid DNA reached the ovules of decapitated florets within 24 hours after its application to the surface of remaining styles at 18 hours after pollination. Plasmid DNA mixed with 1/2 liquid MS medium (Musrashige & Skoog, 1964) containing 4 mg/l NAA and 4 mg/l GA3, plus 0.25% DMSO may have facilitated the entrance of plasmid DNA into onion ovules via the pollen tube pathway. Based on the result of PCR analysis of genomic DNA, 12 to 15% of the plants tested had the 282 bp fragment, the specific portion of the luciferase gene construct. Exogenous DNA should be applied to the decapitated onion florets no later than 18 hour following pollination. Southern blotting of genomic DNA from PCR positive plants indicated that the firefly luciferase gene construct may have been incorporated into the genomic DNA of onion. However, insertion of the foreign DNA in some of the PCR positive plants may have been chimeric since the 282 bp PCR amplified fragment could not be detected at their maturing stages and thereafter (in Allium, cepa cv. DG133), and in some sexually produced progeny (in A fistulosum cv. Heshiko). In addition, expression of the firefly luciferase gene construct was not detected in any of the putative transgenic plants. Some of the PCR positive Heshiko plants, derived from seeds harvested from florets treated with a DNA preparation containing 1/2 liquid MS medium supplied with 4 mg/l NAA, 4 mg/l GA3, still had the 282 bp fragment of the luciferase gene construct in their sexually produced progenies. These results indicated that transformation via the pollen tube pathway has the potential to produce stably transformed onion plants." @default.
• W2279528030 created "2016-06-24" @default.
• W2279528030 creator A5081546172 @default.
• W2279528030 date "1994-05-01" @default.
• W2279528030 modified "2023-09-28" @default.
• W2279528030 title "Investigation of a genetic transformation system in onion" @default.
• W2279528030 cites W1139337119 @default.
• W2279528030 cites W1675584952 @default.
• W2279528030 cites W1969585185 @default.
• W2279528030 cites W1976680054 @default.
• W2279528030 cites W1984887980 @default.
• W2279528030 cites W1987155223 @default.
• W2279528030 cites W1994207549 @default.
• W2279528030 cites W1999627579 @default.
• W2279528030 cites W2001882373 @default.
• W2279528030 cites W2006147026 @default.
• W2279528030 cites W2006807779 @default.
• W2279528030 cites W2021200970 @default.
• W2279528030 cites W2023668652 @default.
• W2279528030 cites W2024556021 @default.
• W2279528030 cites W2029552892 @default.
• W2279528030 cites W2032463086 @default.
• W2279528030 cites W2035745775 @default.
• W2279528030 cites W2040348588 @default.
• W2279528030 cites W2040469547 @default.
• W2279528030 cites W2043153170 @default.
• W2279528030 cites W2046896689 @default.
• W2279528030 cites W2047175573 @default.
• W2279528030 cites W2047683040 @default.
• W2279528030 cites W2050556487 @default.
• W2279528030 cites W2055584669 @default.
• W2279528030 cites W2062706925 @default.
• W2279528030 cites W2063056851 @default.
• W2279528030 cites W2065307188 @default.
• W2279528030 cites W2067018631 @default.
• W2279528030 cites W2074064325 @default.
• W2279528030 cites W2080310611 @default.
• W2279528030 cites W2081877500 @default.
• W2279528030 cites W2082433688 @default.
• W2279528030 cites W2084549017 @default.
• W2279528030 cites W2094463991 @default.
• W2279528030 cites W2097794242 @default.
• W2279528030 cites W2099945740 @default.
• W2279528030 cites W2108739301 @default.
• W2279528030 cites W2132738739 @default.
• W2279528030 cites W2133284568 @default.
• W2279528030 cites W2134812217 @default.
• W2279528030 cites W2155259216 @default.
• W2279528030 cites W2191103488 @default.
• W2279528030 cites W2203478734 @default.
• W2279528030 cites W2212500578 @default.
• W2279528030 cites W2274098256 @default.
• W2279528030 cites W2404591873 @default.
• W2279528030 cites W2460102656 @default.
• W2279528030 cites W2464032107 @default.
• W2279528030 cites W2887356887 @default.
• W2279528030 cites W2892675563 @default.
• W2279528030 cites W2893740065 @default.
• W2279528030 cites W2894440634 @default.
• W2279528030 cites W324835986 @default.
• W2279528030 cites W934641172 @default.
• W2279528030 cites W2273191141 @default.
• W2279528030 hasPublicationYear "1994" @default.
• W2279528030 type Work @default.
• W2279528030 sameAs 2279528030 @default.
• W2279528030 citedByCount "2" @default.
• W2279528030 countsByYear W22795280302016 @default.
• W2279528030 crossrefType "dissertation" @default.
• W2279528030 hasAuthorship W2279528030A5081546172 @default.
• W2279528030 hasConcept C104317684 @default.
• W2279528030 hasConcept C111335760 @default.
• W2279528030 hasConcept C116403925 @default.
• W2279528030 hasConcept C121332964 @default.
• W2279528030 hasConcept C1276947 @default.
• W2279528030 hasConcept C153911025 @default.
• W2279528030 hasConcept C168197293 @default.
• W2279528030 hasConcept C17757408 @default.
• W2279528030 hasConcept C178046730 @default.
• W2279528030 hasConcept C204241405 @default.
• W2279528030 hasConcept C22744801 @default.
• W2279528030 hasConcept C2780618852 @default.
• W2279528030 hasConcept C34069715 @default.
• W2279528030 hasConcept C54009773 @default.
• W2279528030 hasConcept C54355233 @default.
• W2279528030 hasConcept C552990157 @default.
• W2279528030 hasConcept C59822182 @default.
• W2279528030 hasConcept C86803240 @default.
• W2279528030 hasConceptScore W2279528030C104317684 @default.
• W2279528030 hasConceptScore W2279528030C111335760 @default.
• W2279528030 hasConceptScore W2279528030C116403925 @default.
• W2279528030 hasConceptScore W2279528030C121332964 @default.
• W2279528030 hasConceptScore W2279528030C1276947 @default.
• W2279528030 hasConceptScore W2279528030C153911025 @default.
• W2279528030 hasConceptScore W2279528030C168197293 @default.
• W2279528030 hasConceptScore W2279528030C17757408 @default.
• W2279528030 hasConceptScore W2279528030C178046730 @default.
• W2279528030 hasConceptScore W2279528030C204241405 @default.
• W2279528030 hasConceptScore W2279528030C22744801 @default.
• W2279528030 hasConceptScore W2279528030C2780618852 @default.
• W2279528030 hasConceptScore W2279528030C34069715 @default.

• next