FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2279725871> ?p ?o ?g. }

• W2279725871 endingPage "8667" @default.
• W2279725871 startingPage "8664" @default.
• W2279725871 abstract "The S-adenosylmethionine (AdoMet) radical superfamily of enzymes includes over 113,500 unique members, each of which contains one indispensable iron-sulfur (FeS) cluster that is required to generate a 5'-deoxyadenosyl 5'-radical intermediate during catalysis. Enzymes within several subgroups of the superfamily, however, have been found to contain one or more additional FeS clusters. While these additional clusters are absolutely essential for enzyme activity, their exact roles in the function and/or mechanism of action of many of the enzymes are at best speculative, indicating a need to develop methods to characterize and study these clusters in more detail. Here, BtrN, an AdoMet radical dehydrogenase that catalyzes the two-electron oxidation of 2-deoxy-scyllo-inosamine to amino-dideoxy-scyllo-inosose, an intermediate in the biosynthesis of 2-deoxystreptamine antibiotics, is examined through direct electrochemistry, where the potential of both its AdoMet radical and auxiliary [4Fe-4S] clusters can be measured simultaneously. We find that the AdoMet radical cluster exhibits a midpoint potential of -510 mV, while the auxiliary cluster exhibits a midpoint potential of -765 mV, to our knowledge the lowest [4Fe-4S](2+/+) potential to be determined to date. The impact of AdoMet binding and the pH dependence of catalysis are also quantitatively observed. These data show that direct electrochemical methods can be used to further elucidate the chemistry of the burgeoning AdoMet radical superfamily in the future." @default.
• W2279725871 created "2016-06-24" @default.
• W2279725871 creator A5013993666 @default.
• W2279725871 creator A5045998043 @default.
• W2279725871 creator A5055149042 @default.
• W2279725871 creator A5059377097 @default.
• W2279725871 date "2015-06-25" @default.
• W2279725871 modified "2023-09-24" @default.
• W2279725871 title "Electrochemical Resolution of the [4Fe-4S] Centers of the AdoMet Radical Enzyme BtrN: Evidence of Proton Coupling and an Unusual, Low-Potential Auxiliary Cluster" @default.
• W2279725871 cites W1967418107 @default.
• W2279725871 cites W1976406843 @default.
• W2279725871 cites W1976488653 @default.
• W2279725871 cites W1976866213 @default.
• W2279725871 cites W1989980304 @default.
• W2279725871 cites W2000298129 @default.
• W2279725871 cites W2004939146 @default.
• W2279725871 cites W2013828401 @default.
• W2279725871 cites W2025913719 @default.
• W2279725871 cites W2035962761 @default.
• W2279725871 cites W2041705093 @default.
• W2279725871 cites W2050117377 @default.
• W2279725871 cites W2057163298 @default.
• W2279725871 cites W2062920334 @default.
• W2279725871 cites W2068958451 @default.
• W2279725871 cites W2100918802 @default.
• W2279725871 cites W2108639180 @default.
• W2279725871 cites W2123894662 @default.
• W2279725871 cites W2126762016 @default.
• W2279725871 cites W2150710339 @default.
• W2279725871 cites W2317418252 @default.
• W2279725871 cites W2318521608 @default.
• W2279725871 cites W2332418968 @default.
• W2279725871 cites W2396643508 @default.
• W2279725871 doi "https://doi.org/10.1021/jacs.5b03384" @default.
• W2279725871 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26088836" @default.
• W2279725871 hasPublicationYear "2015" @default.
• W2279725871 type Work @default.
• W2279725871 sameAs 2279725871 @default.
• W2279725871 citedByCount "37" @default.
• W2279725871 countsByYear W22797258712016 @default.
• W2279725871 countsByYear W22797258712017 @default.
• W2279725871 countsByYear W22797258712018 @default.
• W2279725871 countsByYear W22797258712019 @default.
• W2279725871 countsByYear W22797258712020 @default.
• W2279725871 countsByYear W22797258712021 @default.
• W2279725871 countsByYear W22797258712022 @default.
• W2279725871 countsByYear W22797258712023 @default.
• W2279725871 crossrefType "journal-article" @default.
• W2279725871 hasAuthorship W2279725871A5013993666 @default.
• W2279725871 hasAuthorship W2279725871A5045998043 @default.
• W2279725871 hasAuthorship W2279725871A5055149042 @default.
• W2279725871 hasAuthorship W2279725871A5059377097 @default.
• W2279725871 hasConcept C112243037 @default.
• W2279725871 hasConcept C145148216 @default.
• W2279725871 hasConcept C147789679 @default.
• W2279725871 hasConcept C161790260 @default.
• W2279725871 hasConcept C164866538 @default.
• W2279725871 hasConcept C170493617 @default.
• W2279725871 hasConcept C17525397 @default.
• W2279725871 hasConcept C178790620 @default.
• W2279725871 hasConcept C181199279 @default.
• W2279725871 hasConcept C185592680 @default.
• W2279725871 hasConcept C199360897 @default.
• W2279725871 hasConcept C21951064 @default.
• W2279725871 hasConcept C24840226 @default.
• W2279725871 hasConcept C2776317432 @default.
• W2279725871 hasConcept C2777852564 @default.
• W2279725871 hasConcept C2779268744 @default.
• W2279725871 hasConcept C41008148 @default.
• W2279725871 hasConcept C52859227 @default.
• W2279725871 hasConcept C55493867 @default.
• W2279725871 hasConcept C71240020 @default.
• W2279725871 hasConcept C92028520 @default.
• W2279725871 hasConcept C96444392 @default.
• W2279725871 hasConceptScore W2279725871C112243037 @default.
• W2279725871 hasConceptScore W2279725871C145148216 @default.
• W2279725871 hasConceptScore W2279725871C147789679 @default.
• W2279725871 hasConceptScore W2279725871C161790260 @default.
• W2279725871 hasConceptScore W2279725871C164866538 @default.
• W2279725871 hasConceptScore W2279725871C170493617 @default.
• W2279725871 hasConceptScore W2279725871C17525397 @default.
• W2279725871 hasConceptScore W2279725871C178790620 @default.
• W2279725871 hasConceptScore W2279725871C181199279 @default.
• W2279725871 hasConceptScore W2279725871C185592680 @default.
• W2279725871 hasConceptScore W2279725871C199360897 @default.
• W2279725871 hasConceptScore W2279725871C21951064 @default.
• W2279725871 hasConceptScore W2279725871C24840226 @default.
• W2279725871 hasConceptScore W2279725871C2776317432 @default.
• W2279725871 hasConceptScore W2279725871C2777852564 @default.
• W2279725871 hasConceptScore W2279725871C2779268744 @default.
• W2279725871 hasConceptScore W2279725871C41008148 @default.
• W2279725871 hasConceptScore W2279725871C52859227 @default.
• W2279725871 hasConceptScore W2279725871C55493867 @default.
• W2279725871 hasConceptScore W2279725871C71240020 @default.
• W2279725871 hasConceptScore W2279725871C92028520 @default.
• W2279725871 hasConceptScore W2279725871C96444392 @default.
• W2279725871 hasFunder F4320337354 @default.
• W2279725871 hasFunder F4320337397 @default.

• next