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- W2279798924 abstract "Duodenal mucosal defense was assessed by measuring blood flow and epithelial intracellular pH (pH i ) of rat proximal duodenum in vivo. Fluorescence microscopy was used to measure epithelial pH i using the trapped, pH i -indicating dye 2′,7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein-AM. Blood flow was measured with laser-Doppler flowmetry. The mucosa was briefly superfused with NH 4 Cl, pH 2.2 buffer, the potent Na + /H + exchange inhibitor 5-( N, N-dimethyl)-amiloride (DMA), or the anion exchange and Na + -[Formula: see text]cotransport inhibitor DIDS. Cryostat sections localized dye fluorescence to the villus tip. Steady-state pH i was 7.02 ± 0.01, which remained stable for 60 min. Interventions that load the cells with protons without affecting superfusate pH (NH 4 Cl prepulse, nigericin with low superfusate K + concentration, DMA, and DIDS) all decreased pH i , supporting our contention that the dye was faithfully measuring pH i . An acid pulse decreased pH i , followed by a DIDS-inhibitable overshoot over baseline. Intracellular acidification increased duodenal blood flow independent of superfusate pH, which was inhibited by DMA, but not by DIDS. We conclude that we have established a novel in vivo microscopy system enabling simultaneous measurements of pH i and blood flow of duodenal epithelium. Na + /H + exchange and Na + -[Formula: see text]cotransport regulate baseline duodenal epithelial pH i . Intracellular acidification enhances duodenal blood flow by a unique, amiloride-inhibitable, superfusate pH-independent mechanism." @default.
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- W2279798924 date "1999-01-01" @default.
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- W2279798924 title "Regulation of intracellular pH and blood flow in rat duodenal epithelium in vivo" @default.
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- W2279798924 doi "https://doi.org/10.1152/ajpgi.1999.276.1.g293" @default.
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