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- W227988006 abstract "This chapter discusses about general relationships between Dalziel coefficients and velocity constants in the random-order two-substrate mechanism. Many dehydrogenases catalyzing two-substrate reactions give linear Lineweaver–Burk plots with respect to each substrate at fixed concentrations of the other, and conform to a Dalziel type of steady-state rate equation. Mathematical analysis shows that Lineweaver–Burk plots in general are nonlinear. The curves obtained may be of different character, but have a common linear asymptote. Experimental observations of the steady-state rate behavior can, for practical reasons, only be carried out over a limited range of substrate concentrations. This range must be considered as narrow in view of the wide range of possible values for velocity constants in the mechanism. The asymptote treatment of linear reciprocal rate data is neither restricted to dehydrogenases, nor to the random-order two-substrate mechanism." @default.
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- W227988006 date "1972-01-01" @default.
- W227988006 modified "2023-09-26" @default.
- W227988006 title "GENERAL RELATIONSHIPS BETWEEN DALZIEL COEFFICIENTS AND VELOCITY CONSTANTS IN THE RANDOM-ORDER TWO-SUBSTRATE MECHANISM" @default.
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- W227988006 doi "https://doi.org/10.1016/b978-0-08-016874-6.50087-4" @default.
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