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• W2280159498 abstract "Vascular endothelial growth factor (VEGF) is an angiogenic cytokine that stimulates the differentiation and function of vascular endothelial cells. VEGF has been implicated in improving nervous system function after injury. However, uncontrolled overexpression of VEGF increases the risk of tumor formation at the site of gene delivery. For this reason, VEGF expression needs to be strictly controlled. The goal of the present study was to understand the effects of hypoxia-induced gene expression system to control VEGF gene expression in neural stem cells (NSCs) on the regeneration of neural tissue after sciatic nerve injury. In this study, we used the erythropoietin (Epo) enhancer-SV40 promoter system (EpoSV-VEGF-NSCs) for hypoxia-specific VEGF expression. We used three types of NSCs: DsRed-NSCs as controls, SV-VEGF-NSCs as uncontrolled VEGF overexpressing NSCs, and EpoSV-VEGF-NSCs. For comparison of VEGF expression at normoxia and hypoxia, we measured the amount of VEGF secreted. VEGF expression decreased at normoxia and increased at hypoxia for EpoSV-VEGF-NSCs; thus, EpoSV-VEGF-NSCs controlled VEGF expression, dependent upon oxygenation condition. To demonstrate the therapeutic effect of EpoSV-VEGF-NSCs, we transplanted each cell line in a neuropathic pain sciatic nerve injury rat model. The transplanted EpoSV-VEGF-NSCs improved sciatic nerve functional index (SFI), mechanical allodynia, and re-myelination similar to the SV-VEGF-NSCs. Additionally, the number of blood vessels increased to a level similar to that of the SV-VEGF-NSCs. However, we did not observe tumor generation in the EpoSV-VEGF-NSC animals that were unlikely to have tumor formation in the SV-VEGF-NSCs. From our results, we determined that EpoSV-VEGF-NSCs safely regulate VEGF gene expression which is dependent upon oxygenation status. In addition, we found that they are therapeutically appropriate for treating sciatic nerve injury." @default.
• W2280159498 created "2016-06-24" @default.
• W2280159498 creator A5019229576 @default.
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• W2280159498 date "2016-03-01" @default.
• W2280159498 modified "2023-10-16" @default.
• W2280159498 title "Hypoxia-specific, VEGF-expressing neural stem cell therapy for safe and effective treatment of neuropathic pain" @default.
• W2280159498 cites W1519112234 @default.
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• W2280159498 cites W1968279831 @default.
• W2280159498 cites W1972409482 @default.
• W2280159498 cites W1973031597 @default.
• W2280159498 cites W1973076149 @default.
• W2280159498 cites W1977213877 @default.
• W2280159498 cites W1978797498 @default.
• W2280159498 cites W1979859079 @default.
• W2280159498 cites W1987522740 @default.
• W2280159498 cites W1987804149 @default.
• W2280159498 cites W1991305397 @default.
• W2280159498 cites W1995078250 @default.
• W2280159498 cites W1996609665 @default.
• W2280159498 cites W2004907588 @default.
• W2280159498 cites W2005846507 @default.
• W2280159498 cites W2017299617 @default.
• W2280159498 cites W2017639172 @default.
• W2280159498 cites W2020041340 @default.
• W2280159498 cites W2020939052 @default.
• W2280159498 cites W2023861393 @default.
• W2280159498 cites W2030492490 @default.
• W2280159498 cites W2032538250 @default.
• W2280159498 cites W2034949619 @default.
• W2280159498 cites W2038863919 @default.
• W2280159498 cites W2039677191 @default.
• W2280159498 cites W2040913522 @default.
• W2280159498 cites W2041804946 @default.
• W2280159498 cites W2042741271 @default.
• W2280159498 cites W2046128464 @default.
• W2280159498 cites W2046440184 @default.
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• W2280159498 cites W2065104606 @default.
• W2280159498 cites W2067212451 @default.
• W2280159498 cites W2071751316 @default.
• W2280159498 cites W2071862338 @default.
• W2280159498 cites W2072839085 @default.
• W2280159498 cites W2076357246 @default.
• W2280159498 cites W2077011385 @default.
• W2280159498 cites W2079692119 @default.
• W2280159498 cites W2081065930 @default.
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• W2280159498 doi "https://doi.org/10.1016/j.jconrel.2016.01.047" @default.
• W2280159498 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26826306" @default.
• W2280159498 hasPublicationYear "2016" @default.
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