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- W2280332364 abstract "We describe a new method to accomplish multiplexed, absolute protein quantification in a targeted fashion. The approach draws upon the recently developed neutron encoding (NeuCode) metabolic labeling strategy and parallel reaction monitoring (PRM). Since PRM scanning relies upon high-resolution tandem mass spectra for targeted protein quantification, incorporation of multiple NeuCode labeled peptides permits high levels of multiplexing that can be accessed from high-resolution tandem mass spectra. Here we demonstrate this approach in cultured cells by monitoring a viral infection and the corresponding viral protein production over many infection time points in a single experiment. In this context the NeuCode PRM combination affords up to 30 channels of quantitative information in a single MS experiment." @default.
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- W2280332364 date "2016-02-25" @default.
- W2280332364 modified "2023-10-18" @default.
- W2280332364 title "Neucode Labels for Multiplexed, Absolute Protein Quantification" @default.
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- W2280332364 doi "https://doi.org/10.1021/acs.analchem.5b04773" @default.
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