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• W2280882287 endingPage "122" @default.
• W2280882287 startingPage "111" @default.
• W2280882287 abstract "AbstractReal-time imaging coupled with a permeabilized cell system presents a very versatile platform to visualize the dynamic and intricate nature of nuclear envelope breakdown, one of the major morphological changes of mitosis. Here, we describe such a strategy in which the plasma membrane of cells expressing fluorescently tagged nucleoporin POM121 and Histone H2B is permeabilized with digitonin. These cells are then incubated with mitotic Xenopus egg extract to create conditions that recapitulate the major events of mitotic nuclear remodeling seen in live-cell imaging, providing the opportunity to probe mechanisms and pathways that coordinate nuclear disassembly.Key wordsPermeabilized cellsLive-imagingNuclear envelope Xenopus egg extractsGFPPOM121Histone 2B mCherry" @default.
• W2280882287 created "2016-06-24" @default.
• W2280882287 creator A5014166749 @default.
• W2280882287 creator A5033573934 @default.
• W2280882287 creator A5055307459 @default.
• W2280882287 date "2012-01-01" @default.
• W2280882287 modified "2023-09-23" @default.
• W2280882287 title "A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown" @default.
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• W2280882287 doi "https://doi.org/10.1007/978-1-62703-056-4_6" @default.
• W2280882287 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4366132" @default.
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