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• W2280979416 abstract "1. The coupling of D2 dopamine receptors and G-proteins has been examined in cholate-solubilized preparations of bovine caudate nucleus. 2. No receptor-G-protein coupling could be detected in solubilized preparations obtained in 0.3% cholate, but if this preparation is diluted 5-fold, coupling is re-established. 3. The dilution process was examined, and it was shown that the change in ionic strength was an important factor in modulating the observed receptor-G-protein interaction. 4. Ionic strength was shown, however, not to be the primary determinant of receptor-G-protein coupling. This is likely to be the formation, upon dilution of the preparation, of vesicles in which receptor and G-protein reassociate. 5. The formation of vesicles upon dilution was examined by a variety of techniques, including thermal-stability studies, gel filtration, centrifugation and electron microscopy." @default.
• W2280979416 created "2016-06-24" @default.
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• W2280979416 date "1992-01-15" @default.
• W2280979416 modified "2023-09-26" @default.
• W2280979416 title "Coupling of D2 dopamine receptors to G-proteins in solubilized preparations of bovine caudate nucleus" @default.
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• W2280979416 doi "https://doi.org/10.1042/bj2810369" @default.
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• W2280979416 hasPublicationYear "1992" @default.
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