Matches in SemOpenAlex for { <https://semopenalex.org/work/W2281189770> ?p ?o ?g. }
- W2281189770 abstract "Nitrogen fixation has been established in protokaryotic model Escherichia coli by transferring a minimal nif gene cluster composed of 9 genes (nifB, nifH, nifD, nifK, nifE, nifN, nifX, hesA and nifV) from Paenibacillus sp. WLY78. However, the nitrogenase activity in the recombinant E. coli 78-7 is only 10 % of that observed in wild-type Paenibacillus. Thus, it is necessary to increase nitrogenase activity through synthetic biology.In order to increase nitrogenase activity in heterologous host, a total of 28 selected genes from Paenibacillus sp. WLY78 and Klebsiella oxytoca were placed under the control of Paenibacillus nif promoter in two different vectors and then they are separately or combinationally transferred to the recombinant E. coli 78-7. Our results demonstrate that Paenibacillus suf operon (Fe-S cluster assembly) and the potential electron transport genes pfoAB, fldA and fer can increase nitrogenase activity. Also, K. oxytoca nifSU (Fe-S cluster assembly) and nifFJ (electron transport specific for nitrogenase) can increase nitrogenase activity. Especially, the combined assembly of the potential Paenibacillus electron transporter genes (pfoABfldA) with K. oxytoca nifSU recovers 50.1 % of wild-type (Paenibacillus) activity. However, K. oxytoca nifWZM and nifQ can not increase activity.The combined assembly of the potential Paenibacillus electron transporter genes (pfoABfldA) with K. oxytoca nifSU recovers 50.1 % of wild-type (Paenibacillus) activity in the recombinant E. coli 78-7. Our results will provide valuable insights for the enhancement of nitrogenase activity in heterogeneous host and will provide guidance for engineering cereal plants with minimal nif genes." @default.
- W2281189770 created "2016-06-24" @default.
- W2281189770 creator A5005172217 @default.
- W2281189770 creator A5016572507 @default.
- W2281189770 creator A5032106989 @default.
- W2281189770 creator A5073567557 @default.
- W2281189770 creator A5085028455 @default.
- W2281189770 date "2016-02-20" @default.
- W2281189770 modified "2023-10-16" @default.
- W2281189770 title "Using synthetic biology to increase nitrogenase activity" @default.
- W2281189770 cites W1485913512 @default.
- W2281189770 cites W1523373466 @default.
- W2281189770 cites W1548708435 @default.
- W2281189770 cites W1595601572 @default.
- W2281189770 cites W1605201659 @default.
- W2281189770 cites W1851952707 @default.
- W2281189770 cites W1937431683 @default.
- W2281189770 cites W1967382476 @default.
- W2281189770 cites W1978687530 @default.
- W2281189770 cites W1983613885 @default.
- W2281189770 cites W1988222859 @default.
- W2281189770 cites W2013410728 @default.
- W2281189770 cites W2017671198 @default.
- W2281189770 cites W2023215942 @default.
- W2281189770 cites W2027406139 @default.
- W2281189770 cites W2028053857 @default.
- W2281189770 cites W2065085840 @default.
- W2281189770 cites W2071279653 @default.
- W2281189770 cites W2075762317 @default.
- W2281189770 cites W2086232077 @default.
- W2281189770 cites W2091957110 @default.
- W2281189770 cites W2096808921 @default.
- W2281189770 cites W2098146748 @default.
- W2281189770 cites W2107855268 @default.
- W2281189770 cites W2109465920 @default.
- W2281189770 cites W2116472408 @default.
- W2281189770 cites W2117430964 @default.
- W2281189770 cites W2125645676 @default.
- W2281189770 cites W2152065014 @default.
- W2281189770 cites W2159216181 @default.
- W2281189770 cites W2169306900 @default.
- W2281189770 cites W2338582510 @default.
- W2281189770 cites W2496680216 @default.
- W2281189770 cites W3212622373 @default.
- W2281189770 cites W859753841 @default.
- W2281189770 doi "https://doi.org/10.1186/s12934-016-0442-6" @default.
- W2281189770 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4761190" @default.
- W2281189770 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26897628" @default.
- W2281189770 hasPublicationYear "2016" @default.
- W2281189770 type Work @default.
- W2281189770 sameAs 2281189770 @default.
- W2281189770 citedByCount "49" @default.
- W2281189770 countsByYear W22811897702016 @default.
- W2281189770 countsByYear W22811897702017 @default.
- W2281189770 countsByYear W22811897702018 @default.
- W2281189770 countsByYear W22811897702019 @default.
- W2281189770 countsByYear W22811897702020 @default.
- W2281189770 countsByYear W22811897702021 @default.
- W2281189770 countsByYear W22811897702022 @default.
- W2281189770 countsByYear W22811897702023 @default.
- W2281189770 crossrefType "journal-article" @default.
- W2281189770 hasAuthorship W2281189770A5005172217 @default.
- W2281189770 hasAuthorship W2281189770A5016572507 @default.
- W2281189770 hasAuthorship W2281189770A5032106989 @default.
- W2281189770 hasAuthorship W2281189770A5073567557 @default.
- W2281189770 hasAuthorship W2281189770A5085028455 @default.
- W2281189770 hasBestOaLocation W22811897701 @default.
- W2281189770 hasConcept C104317684 @default.
- W2281189770 hasConcept C141280058 @default.
- W2281189770 hasConcept C181440489 @default.
- W2281189770 hasConcept C203075996 @default.
- W2281189770 hasConcept C2778050735 @default.
- W2281189770 hasConcept C2778910516 @default.
- W2281189770 hasConcept C2779134421 @default.
- W2281189770 hasConcept C2780696792 @default.
- W2281189770 hasConcept C36857842 @default.
- W2281189770 hasConcept C42062724 @default.
- W2281189770 hasConcept C523546767 @default.
- W2281189770 hasConcept C54355233 @default.
- W2281189770 hasConcept C547475151 @default.
- W2281189770 hasConcept C55493867 @default.
- W2281189770 hasConcept C86803240 @default.
- W2281189770 hasConcept C89423630 @default.
- W2281189770 hasConceptScore W2281189770C104317684 @default.
- W2281189770 hasConceptScore W2281189770C141280058 @default.
- W2281189770 hasConceptScore W2281189770C181440489 @default.
- W2281189770 hasConceptScore W2281189770C203075996 @default.
- W2281189770 hasConceptScore W2281189770C2778050735 @default.
- W2281189770 hasConceptScore W2281189770C2778910516 @default.
- W2281189770 hasConceptScore W2281189770C2779134421 @default.
- W2281189770 hasConceptScore W2281189770C2780696792 @default.
- W2281189770 hasConceptScore W2281189770C36857842 @default.
- W2281189770 hasConceptScore W2281189770C42062724 @default.
- W2281189770 hasConceptScore W2281189770C523546767 @default.
- W2281189770 hasConceptScore W2281189770C54355233 @default.
- W2281189770 hasConceptScore W2281189770C547475151 @default.
- W2281189770 hasConceptScore W2281189770C55493867 @default.
- W2281189770 hasConceptScore W2281189770C86803240 @default.
- W2281189770 hasConceptScore W2281189770C89423630 @default.
- W2281189770 hasIssue "1" @default.