Matches in SemOpenAlex for { <https://semopenalex.org/work/W2281365953> ?p ?o ?g. }
- W2281365953 endingPage "91" @default.
- W2281365953 startingPage "84" @default.
- W2281365953 abstract "Abstract Orchid seeds are among the smallest seeds in nature and they are naturally rich in fatty acids. However, the fatty acid composition of orchid seeds has not been investigated because the sample masses utilized for widely used methods for fatty acid profiling would generally require prohibitively large numbers (i.e. 10,000s) of seeds. The present work aimed to develop a method for fatty acid analysis using gas chromatography–mass spectrometry on small quantities (mg) of seeds. The method was developed using the seeds of two species, Dactylorhiza fuchsii , a temperate terrestrial, and Grammatophyllum speciosum , a tropical epiphyte. A range of sample masses was tested to determine the minimum mass required to achieve reliable fatty acid composition data. A direct transesterification method was used, which did not require extraction of fatty acids from seeds prior to analysis, and the effects of seed processing (crushed versus intact seeds) and incubation time in toluene on fatty acid yield were tested. Stable fatty acid profiles were obtained using as little as 10 mg of seeds. Neither crushing the seeds nor extending the toluene incubation step had much effect on the fatty acid yield. The simple direct transesterification method presented will enable the fatty acid composition of orchid seeds, and possibly other small seeds, to be determined reliably for studies into seed development, storage and germination." @default.
- W2281365953 created "2016-06-24" @default.
- W2281365953 creator A5006026456 @default.
- W2281365953 creator A5019274860 @default.
- W2281365953 creator A5040417301 @default.
- W2281365953 creator A5068070356 @default.
- W2281365953 creator A5089540590 @default.
- W2281365953 date "2015-12-22" @default.
- W2281365953 modified "2023-09-27" @default.
- W2281365953 title "Development of a reliable GC-MS method for fatty acid profiling using direct transesterification of minimal quantities of microscopic orchid seeds" @default.
- W2281365953 cites W1686139248 @default.
- W2281365953 cites W1933801203 @default.
- W2281365953 cites W1965495607 @default.
- W2281365953 cites W1969963770 @default.
- W2281365953 cites W1973313212 @default.
- W2281365953 cites W1973732173 @default.
- W2281365953 cites W1973734054 @default.
- W2281365953 cites W1975804586 @default.
- W2281365953 cites W1977820217 @default.
- W2281365953 cites W1979739949 @default.
- W2281365953 cites W1981137773 @default.
- W2281365953 cites W1990096841 @default.
- W2281365953 cites W1996358811 @default.
- W2281365953 cites W2023108677 @default.
- W2281365953 cites W2024556021 @default.
- W2281365953 cites W2037685791 @default.
- W2281365953 cites W2039935451 @default.
- W2281365953 cites W2045979248 @default.
- W2281365953 cites W2049125173 @default.
- W2281365953 cites W2055538466 @default.
- W2281365953 cites W2063878611 @default.
- W2281365953 cites W2065685042 @default.
- W2281365953 cites W2078453477 @default.
- W2281365953 cites W2085715664 @default.
- W2281365953 cites W2095166254 @default.
- W2281365953 cites W2102699103 @default.
- W2281365953 cites W2115631298 @default.
- W2281365953 cites W2117664566 @default.
- W2281365953 cites W2118935778 @default.
- W2281365953 cites W2121181291 @default.
- W2281365953 cites W2150847687 @default.
- W2281365953 cites W2152065163 @default.
- W2281365953 cites W2152311269 @default.
- W2281365953 cites W2153269730 @default.
- W2281365953 cites W2168003071 @default.
- W2281365953 cites W2174021099 @default.
- W2281365953 cites W2211467290 @default.
- W2281365953 cites W2320905403 @default.
- W2281365953 cites W2335022749 @default.
- W2281365953 cites W4254346144 @default.
- W2281365953 cites W98036129 @default.
- W2281365953 doi "https://doi.org/10.1017/s0960258515000367" @default.
- W2281365953 hasPublicationYear "2015" @default.
- W2281365953 type Work @default.
- W2281365953 sameAs 2281365953 @default.
- W2281365953 citedByCount "6" @default.
- W2281365953 countsByYear W22813659532017 @default.
- W2281365953 countsByYear W22813659532019 @default.
- W2281365953 countsByYear W22813659532020 @default.
- W2281365953 countsByYear W22813659532021 @default.
- W2281365953 countsByYear W22813659532022 @default.
- W2281365953 crossrefType "journal-article" @default.
- W2281365953 hasAuthorship W2281365953A5006026456 @default.
- W2281365953 hasAuthorship W2281365953A5019274860 @default.
- W2281365953 hasAuthorship W2281365953A5040417301 @default.
- W2281365953 hasAuthorship W2281365953A5068070356 @default.
- W2281365953 hasAuthorship W2281365953A5089540590 @default.
- W2281365953 hasConcept C100701293 @default.
- W2281365953 hasConcept C161790260 @default.
- W2281365953 hasConcept C162356407 @default.
- W2281365953 hasConcept C178790620 @default.
- W2281365953 hasConcept C185592680 @default.
- W2281365953 hasConcept C205345274 @default.
- W2281365953 hasConcept C2777241282 @default.
- W2281365953 hasConcept C2779607525 @default.
- W2281365953 hasConcept C2780209386 @default.
- W2281365953 hasConcept C31903555 @default.
- W2281365953 hasConcept C43617362 @default.
- W2281365953 hasConcept C52896960 @default.
- W2281365953 hasConcept C543025807 @default.
- W2281365953 hasConcept C55493867 @default.
- W2281365953 hasConcept C59822182 @default.
- W2281365953 hasConcept C86803240 @default.
- W2281365953 hasConceptScore W2281365953C100701293 @default.
- W2281365953 hasConceptScore W2281365953C161790260 @default.
- W2281365953 hasConceptScore W2281365953C162356407 @default.
- W2281365953 hasConceptScore W2281365953C178790620 @default.
- W2281365953 hasConceptScore W2281365953C185592680 @default.
- W2281365953 hasConceptScore W2281365953C205345274 @default.
- W2281365953 hasConceptScore W2281365953C2777241282 @default.
- W2281365953 hasConceptScore W2281365953C2779607525 @default.
- W2281365953 hasConceptScore W2281365953C2780209386 @default.
- W2281365953 hasConceptScore W2281365953C31903555 @default.
- W2281365953 hasConceptScore W2281365953C43617362 @default.
- W2281365953 hasConceptScore W2281365953C52896960 @default.
- W2281365953 hasConceptScore W2281365953C543025807 @default.
- W2281365953 hasConceptScore W2281365953C55493867 @default.
- W2281365953 hasConceptScore W2281365953C59822182 @default.