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- W2281633345 abstract "To investigate if Alternaria proteases participate in the direct activation of airway epithelial cells, we are exploring a possible role for protease activated receptor type 2 (PAR-2). We stimulated 16HBE140- cells with 7.5 μg/ml Alternaria culture filtrate (Alt), 100μM PAR-2 agonist, media alone or Alt depleted of proteases by heat treatment (70°C 30min; heated Alt) for 3, 6 and 12 hours. After stimulations, supernatants were collected, frozen and batch assayed for cytokines (ENA78, FGFbasic, VEGF, IL1α, IL1ra, GMCSF, GCSF, IL6, IL8, TNF-α and RANTES) by Luminex. At the 3 hour time, both Alt and PAR-2 agonist increased production of GMCSF, VEGF, IL6, TNF-α and IL8. At 6 hours, constitutive ENA-78 production was inhibited by Alt but was increased by PAR-2 agonist. At both 6 and 12 hr, GMCSF, GCSF,VEGF, IL6 and TNF-α were increased by both stimulants. Stimulation with heated Alt significantly reduced GCSF, IL1α, IL-6, IL-8, TNF-α. The reduction in these inflammatory cytokines by heated Alt suggests that the presence of proteases is necessary for their production by Alt. The similarities demonstrated for GCSF, IL1α, IL-6, IL-8 and TNF-α production by Alt and PAR-2 agonist and their reduction by heated Alt support the involvement of PAR-2 in Alt activation of airway epithelial cells, whereas IL1ra, GMCSF and FGFbasic production and the inhibition of ENA-78 and RANTES may occur via other pathways. Funded in part by Grants HL56177, AI42268, AI61811" @default.
- W2281633345 created "2016-06-24" @default.
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- W2281633345 date "2010-04-01" @default.
- W2281633345 modified "2023-09-27" @default.
- W2281633345 title "PAR‐2 involvement in activation of human airway epithelial cells" @default.
- W2281633345 doi "https://doi.org/10.1096/fasebj.24.1_supplement.970.3" @default.
- W2281633345 hasPublicationYear "2010" @default.
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