FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2282056688> ?p ?o ?g. }

• W2282056688 endingPage "10649" @default.
• W2282056688 startingPage "10638" @default.
• W2282056688 abstract "Chimeric antigen receptor (CAR) transduced T cells have been used to efficiently kill the target tumor cells depending on the single chain variable fragment (scFv) against the specific tumor associated antigen. Here we show the high specific cytotoxicity of the CAR-T cells with very low effector to target cell (E:T) ratio owing to the CD19-scFv, which was constructed in our laboratory and proved to be highly effective in our previous study. Four plasmids containing three generation of CAR were constructed by cloning the CD19-CAR fragment into the lentiviral vector pCDH. CD3 positive T cells were successfully transduced and the CAR protein expression was confirmed by flow cytometry and Western blot. When cocultured with CD19 positive leukemia cell line Nalm-6 cells, CAR-T cells showed specific cytotoxicity: the percentage of target cells decreased to 0 in 24 hours; IL-2, IFN-γ and TNF-α produced in cocultured supernatants increased obviously; and the cytotoxicity reached more than 80%, still remarkable even when the E:T ratio was as low as 1:4. Dynamic change of cell interaction between CAR-T and leukemia cells was visually tracked by using living cells workstation for the first time. A NOD/SCID B-ALL murine model was established using Nalm-6 cells inoculation with a morbidity rate of 100%, and the survival time was prolonged statistically with CAR-T cell treatment. These data demonstrate that the CAR-T cells we prepared could be a promising treatment strategy for CD19 positive tumor diseases." @default.
• W2282056688 created "2016-06-24" @default.
• W2282056688 creator A5015102287 @default.
• W2282056688 creator A5025769722 @default.
• W2282056688 creator A5043567666 @default.
• W2282056688 creator A5044625906 @default.
• W2282056688 creator A5047736510 @default.
• W2282056688 creator A5080605140 @default.
• W2282056688 creator A5086214965 @default.
• W2282056688 creator A5090438720 @default.
• W2282056688 creator A5091284530 @default.
• W2282056688 date "2016-01-30" @default.
• W2282056688 modified "2023-09-26" @default.
• W2282056688 title "Construction of a new anti-CD19 chimeric antigen receptor and the anti-leukemia function study of the transduced T cells" @default.
• W2282056688 cites W1555553634 @default.
• W2282056688 cites W1975999249 @default.
• W2282056688 cites W1979818745 @default.
• W2282056688 cites W1982795546 @default.
• W2282056688 cites W1987446594 @default.
• W2282056688 cites W1989485300 @default.
• W2282056688 cites W2001297737 @default.
• W2282056688 cites W2023068069 @default.
• W2282056688 cites W2028460943 @default.
• W2282056688 cites W2037036587 @default.
• W2282056688 cites W2045475405 @default.
• W2282056688 cites W2047205338 @default.
• W2282056688 cites W2051549641 @default.
• W2282056688 cites W2078783659 @default.
• W2282056688 cites W2079766756 @default.
• W2282056688 cites W2085533078 @default.
• W2282056688 cites W2099570622 @default.
• W2282056688 cites W2104187048 @default.
• W2282056688 cites W2105400883 @default.
• W2282056688 cites W21144364 @default.
• W2282056688 cites W2118796952 @default.
• W2282056688 cites W2129702476 @default.
• W2282056688 cites W2130011407 @default.
• W2282056688 cites W2130122821 @default.
• W2282056688 cites W2130338247 @default.
• W2282056688 cites W2146059841 @default.
• W2282056688 cites W2155569291 @default.
• W2282056688 cites W2167550136 @default.
• W2282056688 cites W4245362842 @default.
• W2282056688 doi "https://doi.org/10.18632/oncotarget.7079" @default.
• W2282056688 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4891147" @default.
• W2282056688 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26840021" @default.
• W2282056688 hasPublicationYear "2016" @default.
• W2282056688 type Work @default.
• W2282056688 sameAs 2282056688 @default.
• W2282056688 citedByCount "29" @default.
• W2282056688 countsByYear W22820566882016 @default.
• W2282056688 countsByYear W22820566882017 @default.
• W2282056688 countsByYear W22820566882018 @default.
• W2282056688 countsByYear W22820566882019 @default.
• W2282056688 countsByYear W22820566882020 @default.
• W2282056688 countsByYear W22820566882021 @default.
• W2282056688 countsByYear W22820566882022 @default.
• W2282056688 countsByYear W22820566882023 @default.
• W2282056688 crossrefType "journal-article" @default.
• W2282056688 hasAuthorship W2282056688A5015102287 @default.
• W2282056688 hasAuthorship W2282056688A5025769722 @default.
• W2282056688 hasAuthorship W2282056688A5043567666 @default.
• W2282056688 hasAuthorship W2282056688A5044625906 @default.
• W2282056688 hasAuthorship W2282056688A5047736510 @default.
• W2282056688 hasAuthorship W2282056688A5080605140 @default.
• W2282056688 hasAuthorship W2282056688A5086214965 @default.
• W2282056688 hasAuthorship W2282056688A5090438720 @default.
• W2282056688 hasAuthorship W2282056688A5091284530 @default.
• W2282056688 hasBestOaLocation W22820566881 @default.
• W2282056688 hasConcept C109316439 @default.
• W2282056688 hasConcept C147483822 @default.
• W2282056688 hasConcept C153911025 @default.
• W2282056688 hasConcept C154317977 @default.
• W2282056688 hasConcept C202751555 @default.
• W2282056688 hasConcept C203014093 @default.
• W2282056688 hasConcept C2776090121 @default.
• W2282056688 hasConcept C2778461978 @default.
• W2282056688 hasConcept C2778957590 @default.
• W2282056688 hasConcept C3875195 @default.
• W2282056688 hasConcept C502942594 @default.
• W2282056688 hasConcept C553184892 @default.
• W2282056688 hasConcept C55493867 @default.
• W2282056688 hasConcept C86803240 @default.
• W2282056688 hasConcept C8891405 @default.
• W2282056688 hasConceptScore W2282056688C109316439 @default.
• W2282056688 hasConceptScore W2282056688C147483822 @default.
• W2282056688 hasConceptScore W2282056688C153911025 @default.
• W2282056688 hasConceptScore W2282056688C154317977 @default.
• W2282056688 hasConceptScore W2282056688C202751555 @default.
• W2282056688 hasConceptScore W2282056688C203014093 @default.
• W2282056688 hasConceptScore W2282056688C2776090121 @default.
• W2282056688 hasConceptScore W2282056688C2778461978 @default.
• W2282056688 hasConceptScore W2282056688C2778957590 @default.
• W2282056688 hasConceptScore W2282056688C3875195 @default.
• W2282056688 hasConceptScore W2282056688C502942594 @default.
• W2282056688 hasConceptScore W2282056688C553184892 @default.
• W2282056688 hasConceptScore W2282056688C55493867 @default.
• W2282056688 hasConceptScore W2282056688C86803240 @default.

• next