Matches in SemOpenAlex for { <https://semopenalex.org/work/W2282157589> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2282157589 endingPage "449" @default.
- W2282157589 startingPage "443" @default.
- W2282157589 abstract "The gene encoding an alpha-L-arabinofuranosidase (abfA) was homologously cloned in Streptomyces lividans and its DNA sequence was determined. The enzyme was purified from the cytoplasm of the hyperproducing clone S. lividans IAF116. Its M(r) was estimated by gel filtration and found to be approx. 380,000. Since SDS/PAGE indicated a native protein of M(r) 69,000, it can be concluded that the native protein consists of several subunits of that size. The pI value was 4.6. The kinetic constants determined with p-nitrophenyl alpha-L-arabinofuranoside as substrate were a Vmax of 180 units/mg of protein and a Km of 0.6 mM. The specific activity of the purified enzyme on this substrate was 153 units/mg of protein. Optimal enzyme activity was obtained at 60 degrees C and pH 6.0. The enzyme cleaved p-nitrophenyl alpha-L-arabinofuranoside, but had no activity on a variety of other p-nitrophenyl glycosides, except on p-nitrophenyl beta-D-xylopyranoside. The enzyme showed no activity on oat-spelts (Avena sativa) xylan or arabinogalactan, but acted on beet (Beta) arabinan or arabinoxylan. Hydrolysis occurred on arabino-oligoxylosides obtained from oat-splets xylan after digestion with xylanases. Since S. lividans normally does not secrete arabinofuranosidase, this enzyme may play a role in the assimilation of arabinose moieties from arabinose-containing xylo-oligosaccharides generated by beta-xylosidases or xylanases." @default.
- W2282157589 created "2016-06-24" @default.
- W2282157589 creator A5036037468 @default.
- W2282157589 creator A5064972005 @default.
- W2282157589 creator A5068110957 @default.
- W2282157589 creator A5090791173 @default.
- W2282157589 date "1994-09-01" @default.
- W2282157589 modified "2023-10-16" @default.
- W2282157589 title "Purification and characterization of an α-<scp>l</scp>-arabinofuranosidase from <i>Streptomyces lividans</i> 66 and DNA sequence of the gene (<i>abfA</i>)" @default.
- W2282157589 cites W1521148011 @default.
- W2282157589 cites W1543448283 @default.
- W2282157589 cites W1555786425 @default.
- W2282157589 cites W1558135144 @default.
- W2282157589 cites W1568459291 @default.
- W2282157589 cites W1570791889 @default.
- W2282157589 cites W1775749144 @default.
- W2282157589 cites W1897865063 @default.
- W2282157589 cites W1992743134 @default.
- W2282157589 cites W1992743836 @default.
- W2282157589 cites W1998058913 @default.
- W2282157589 cites W2000753254 @default.
- W2282157589 cites W2007694112 @default.
- W2282157589 cites W2056559984 @default.
- W2282157589 cites W2075856692 @default.
- W2282157589 cites W2091153733 @default.
- W2282157589 cites W2129133023 @default.
- W2282157589 cites W2138270253 @default.
- W2282157589 cites W2152431828 @default.
- W2282157589 cites W2168553434 @default.
- W2282157589 cites W2284822 @default.
- W2282157589 cites W299204690 @default.
- W2282157589 doi "https://doi.org/10.1042/bj3020443" @default.
- W2282157589 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1137248" @default.
- W2282157589 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8092996" @default.
- W2282157589 hasPublicationYear "1994" @default.
- W2282157589 type Work @default.
- W2282157589 sameAs 2282157589 @default.
- W2282157589 citedByCount "46" @default.
- W2282157589 countsByYear W22821575892012 @default.
- W2282157589 countsByYear W22821575892013 @default.
- W2282157589 countsByYear W22821575892014 @default.
- W2282157589 countsByYear W22821575892015 @default.
- W2282157589 countsByYear W22821575892017 @default.
- W2282157589 countsByYear W22821575892020 @default.
- W2282157589 countsByYear W22821575892021 @default.
- W2282157589 countsByYear W22821575892023 @default.
- W2282157589 crossrefType "journal-article" @default.
- W2282157589 hasAuthorship W2282157589A5036037468 @default.
- W2282157589 hasAuthorship W2282157589A5064972005 @default.
- W2282157589 hasAuthorship W2282157589A5068110957 @default.
- W2282157589 hasAuthorship W2282157589A5090791173 @default.
- W2282157589 hasBestOaLocation W22821575892 @default.
- W2282157589 hasConcept C100544194 @default.
- W2282157589 hasConcept C100817775 @default.
- W2282157589 hasConcept C153911025 @default.
- W2282157589 hasConcept C181199279 @default.
- W2282157589 hasConcept C185592680 @default.
- W2282157589 hasConcept C2776084176 @default.
- W2282157589 hasConcept C2778691952 @default.
- W2282157589 hasConcept C2780294150 @default.
- W2282157589 hasConcept C28212737 @default.
- W2282157589 hasConcept C55493867 @default.
- W2282157589 hasConcept C86803240 @default.
- W2282157589 hasConceptScore W2282157589C100544194 @default.
- W2282157589 hasConceptScore W2282157589C100817775 @default.
- W2282157589 hasConceptScore W2282157589C153911025 @default.
- W2282157589 hasConceptScore W2282157589C181199279 @default.
- W2282157589 hasConceptScore W2282157589C185592680 @default.
- W2282157589 hasConceptScore W2282157589C2776084176 @default.
- W2282157589 hasConceptScore W2282157589C2778691952 @default.
- W2282157589 hasConceptScore W2282157589C2780294150 @default.
- W2282157589 hasConceptScore W2282157589C28212737 @default.
- W2282157589 hasConceptScore W2282157589C55493867 @default.
- W2282157589 hasConceptScore W2282157589C86803240 @default.
- W2282157589 hasIssue "2" @default.
- W2282157589 hasLocation W22821575891 @default.
- W2282157589 hasLocation W22821575892 @default.
- W2282157589 hasLocation W22821575893 @default.
- W2282157589 hasLocation W22821575894 @default.
- W2282157589 hasOpenAccess W2282157589 @default.
- W2282157589 hasPrimaryLocation W22821575891 @default.
- W2282157589 hasRelatedWork W1976314208 @default.
- W2282157589 hasRelatedWork W1988259208 @default.
- W2282157589 hasRelatedWork W1988346978 @default.
- W2282157589 hasRelatedWork W2010372900 @default.
- W2282157589 hasRelatedWork W2064042198 @default.
- W2282157589 hasRelatedWork W2064587910 @default.
- W2282157589 hasRelatedWork W2079358172 @default.
- W2282157589 hasRelatedWork W2101908377 @default.
- W2282157589 hasRelatedWork W2113606128 @default.
- W2282157589 hasRelatedWork W412475053 @default.
- W2282157589 hasVolume "302" @default.
- W2282157589 isParatext "false" @default.
- W2282157589 isRetracted "false" @default.
- W2282157589 magId "2282157589" @default.
- W2282157589 workType "article" @default.