Matches in SemOpenAlex for { <https://semopenalex.org/work/W2282201428> ?p ?o ?g. }
- W2282201428 abstract "Epithelial-mesenchymal transition (EMT) is associated with altered connection and junctions between cells and changes in abilities of invasion and migration. In this study, we investigated whether SK-BR-3 breast cancer cells induced to undergo EMT exhibit changes in morphological and invasion abilities after Transforming growth factor β1 (TGF-β1) treatment. Serum-deprived SK-BR-3 cells were treated with TGF-β1 (0, 10 ng/mL) for 24 h. The cells morphological changes were observed and imaged using inverted phase contrast microscope. Scratch experiment and invasion experiment were employed to detect changes of invasion ability, cell-flow experiment was used to assess cell cycle, immunohistochemistry technique was used to detect epithelial and mesenchymal markers after the crawling cells were fixed. Our research reveal that SK-BR-3 cells become larger and more messy, the elongated cells extend pseudopodia, the link of the cells became more loosely and cell gap widened after TGF-β1 treatment. SK-BR-3 cells showed faster growing and improved invasion abilities after TGF-β1 treatment, and reduced G1 phase cells proportion in the total number of cells after the conversion, in contrast the S phase cells accounted for the proportion of the total number of cells increased. These findings indicate that TGF-β1-induced EMT in breast cancer cells may be associated with major alterations in morphological and invasion abilities." @default.
- W2282201428 created "2016-06-24" @default.
- W2282201428 creator A5001085160 @default.
- W2282201428 creator A5004125829 @default.
- W2282201428 creator A5010737776 @default.
- W2282201428 creator A5015695812 @default.
- W2282201428 creator A5023460004 @default.
- W2282201428 creator A5024016708 @default.
- W2282201428 creator A5067701301 @default.
- W2282201428 creator A5076903553 @default.
- W2282201428 creator A5077860517 @default.
- W2282201428 creator A5086101087 @default.
- W2282201428 creator A5089165046 @default.
- W2282201428 date "2014-01-01" @default.
- W2282201428 modified "2023-09-28" @default.
- W2282201428 title "Transforming growth factor-β1 regulates epithelial-mesenchymal transition in association with cancer stem-like cells in a breast cancer cell line." @default.
- W2282201428 cites W1542686017 @default.
- W2282201428 cites W1833059567 @default.
- W2282201428 cites W1963706432 @default.
- W2282201428 cites W1966097141 @default.
- W2282201428 cites W1967767010 @default.
- W2282201428 cites W1975668484 @default.
- W2282201428 cites W1978192120 @default.
- W2282201428 cites W1984434134 @default.
- W2282201428 cites W1990339592 @default.
- W2282201428 cites W1993254680 @default.
- W2282201428 cites W2000953544 @default.
- W2282201428 cites W2004939067 @default.
- W2282201428 cites W2005326754 @default.
- W2282201428 cites W2007023713 @default.
- W2282201428 cites W2022487233 @default.
- W2282201428 cites W2033826636 @default.
- W2282201428 cites W2037463258 @default.
- W2282201428 cites W2038403939 @default.
- W2282201428 cites W2038416557 @default.
- W2282201428 cites W2044600584 @default.
- W2282201428 cites W2066226023 @default.
- W2282201428 cites W2067311782 @default.
- W2282201428 cites W2070295378 @default.
- W2282201428 cites W2079331411 @default.
- W2282201428 cites W2082109120 @default.
- W2282201428 cites W2086555983 @default.
- W2282201428 cites W2110014936 @default.
- W2282201428 cites W2121562319 @default.
- W2282201428 cites W2125505694 @default.
- W2282201428 cites W2131011879 @default.
- W2282201428 cites W2134781728 @default.
- W2282201428 cites W2145445766 @default.
- W2282201428 cites W2145711775 @default.
- W2282201428 cites W2150186781 @default.
- W2282201428 cites W2153360267 @default.
- W2282201428 cites W2157957776 @default.
- W2282201428 cites W2164358503 @default.
- W2282201428 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4057834" @default.
- W2282201428 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24955155" @default.
- W2282201428 hasPublicationYear "2014" @default.
- W2282201428 type Work @default.
- W2282201428 sameAs 2282201428 @default.
- W2282201428 citedByCount "4" @default.
- W2282201428 countsByYear W22822014282015 @default.
- W2282201428 countsByYear W22822014282016 @default.
- W2282201428 crossrefType "journal-article" @default.
- W2282201428 hasAuthorship W2282201428A5001085160 @default.
- W2282201428 hasAuthorship W2282201428A5004125829 @default.
- W2282201428 hasAuthorship W2282201428A5010737776 @default.
- W2282201428 hasAuthorship W2282201428A5015695812 @default.
- W2282201428 hasAuthorship W2282201428A5023460004 @default.
- W2282201428 hasAuthorship W2282201428A5024016708 @default.
- W2282201428 hasAuthorship W2282201428A5067701301 @default.
- W2282201428 hasAuthorship W2282201428A5076903553 @default.
- W2282201428 hasAuthorship W2282201428A5077860517 @default.
- W2282201428 hasAuthorship W2282201428A5086101087 @default.
- W2282201428 hasAuthorship W2282201428A5089165046 @default.
- W2282201428 hasConcept C118131993 @default.
- W2282201428 hasConcept C121608353 @default.
- W2282201428 hasConcept C126322002 @default.
- W2282201428 hasConcept C142724271 @default.
- W2282201428 hasConcept C1491633281 @default.
- W2282201428 hasConcept C198826908 @default.
- W2282201428 hasConcept C199528912 @default.
- W2282201428 hasConcept C2779013556 @default.
- W2282201428 hasConcept C29537977 @default.
- W2282201428 hasConcept C502942594 @default.
- W2282201428 hasConcept C530470458 @default.
- W2282201428 hasConcept C54355233 @default.
- W2282201428 hasConcept C55427017 @default.
- W2282201428 hasConcept C55493867 @default.
- W2282201428 hasConcept C71924100 @default.
- W2282201428 hasConcept C76419328 @default.
- W2282201428 hasConcept C81885089 @default.
- W2282201428 hasConcept C86803240 @default.
- W2282201428 hasConcept C95444343 @default.
- W2282201428 hasConcept C96232424 @default.
- W2282201428 hasConceptScore W2282201428C118131993 @default.
- W2282201428 hasConceptScore W2282201428C121608353 @default.
- W2282201428 hasConceptScore W2282201428C126322002 @default.
- W2282201428 hasConceptScore W2282201428C142724271 @default.
- W2282201428 hasConceptScore W2282201428C1491633281 @default.
- W2282201428 hasConceptScore W2282201428C198826908 @default.
- W2282201428 hasConceptScore W2282201428C199528912 @default.