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- W2282209991 abstract "Objective:To investigate folic acid receptor-mediated targeted gene delivery of chitosan-pGPU6/GFP/Neo nanoparticles to tumor cells expressing different degrees of folic acid receptors.Methods: Folate-chitosan-pGPU6/GFP/Neo nanoparticles(FA-CS-DNAnano) and chitosan-pGPU6/GFP/Neo nanoparticles(CS-DNAnano) were prepared and identified by infrared spectrometer,and their morphology characteristics and diameters were observed under transmission electron microscope.Human ovarian cancer cell line SKOV3,breast cancer cell line MCF-7,and cervical cancer line HeLa were transfected with the prepared nanoparticles;the transfection efficiency was evaluated by flow cytometry;and cytotoxicities of the nanoparticles were detected by MTT method.Results: FA-CS-DNAnano and CS-DNAnano were successfully prepared.The particles had a smooth surface and a uniform structure,with the diameters being(78.1±0.3) nm and(138.4±0.7) nm.Transfection efficiency of FA-CS-DNAnano was significantly higher than that of CS-DNAnano in SKOV3 and MCF-7 cells([24.3±0.7]% vs [0.7±0.1]%,[16.8±1.2]% vs [0.3±0.1]%;all P0.01),but not in HeLa cells(P0.05).The cell vitalities of MCF-7,SKOV3 and HeLa cells were(87.9±2.4)%,(91.4±1.0)%,(97.4±1.1)% before transfection,and were(63.0±2.5)%,(90.6±1.3)%,and(99.3±1.6)% after transfection with FA-CS-DNAnano.Conclusion: Chitosan modified with folic acid is an efficient target gene carrier for folic acid receptor highly positive SKOV3 and MCF-7 tumor cells." @default.
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- W2282209991 date "2010-01-01" @default.
- W2282209991 modified "2023-09-24" @default.
- W2282209991 title "Folic acid receptor-mediated targeted transfection of chitosan-pGPU6/GEP/Neo nanoparticles into tumor cells." @default.
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