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- W2282879586 abstract "In order to investigate the effect of the sugar composition of the medium on IgG glycosylation in mouse hybridoma cultivation, the batch culture of mouse hybridoma 3A21 (RCB 1285) was carried out under various sugar compositions. The macroheterogeneity (relative ratio of glycosylated antibody) was analyzed by an enzyme-linked lectin binding assay during cultivation. The relative ratio of glycosylated antibody decreased as cultivation progressed. However, in mannoseenriched cultivation, the relative ratio was maintained at a high level. The microheterogeneity of each oligosaccharide chain was analyzed by two-dimensional mapping techniques. The high-mannose type oligosaccharide chain content was high in mannose-enriched cultivation The content of oligosaccharide chains terminating in galactose residues, which plays an important role in the immune system, was shown to be high in galactose-enriched cultivation. Using galactose- and mannoseenriched cultivations, the number of oligosaccharide chains terminating in galactose residues could be increased." @default.
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- W2282879586 date "2005-11-30" @default.
- W2282879586 modified "2023-10-18" @default.
- W2282879586 title "Effect of Sugar Composition on the Heterogeneity of Antibod in Hybridoma Cultivation" @default.
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- W2282879586 cites W1983763081 @default.
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- W2282879586 doi "https://doi.org/10.1007/0-306-46869-7_25" @default.
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