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• W2283346859 abstract "The aim of this study was to investigate whether the genetic variants of apolipoprotein A1: MspI polymorphisms in non translated region at -75bp upstream and +83bp in the first intron, had any impact on the development of CAD. A total of 400 unrelated adult subjects were enrolled in the study (200 CAD patients & 200 CAD-free controls) from the Kashmir region. Plasma levels of lipids were estimated for each sample by using photometric system. DNA extracted from blood samples was amplified by means of polymerase chain reaction, and then subjected to RFLP by using MspI enzyme; resolving the product on 3% agarose gel stained with ethidium bromide, and visualized under UV light. The G →A substitution at –75bp in apo-A1 revealed that GG genotype was more prevalent among cases, whereas GA and AA was more prevalent among controls, however the difference was insignificant (p=0.145). Overall ‘G’ allele was more prevalent than ‘A’ allele among the study population but the difference was again insignificant (p=0.0106). At +83bp site, there was complete absence of ‘T’ allele both in cases and controls in apoA1, so that no individual for genotypes C/T and T/T at apo-A1 gene was found. Instead the ‘C’ allele, in homozygous dominant condition (C/C), was present throughout (100%) the study population. From our study, it can be concluded that polymorphism at -75bp (G/A) and +83bp (C/T) polymorphism of Apo-A1 gene appears to have no apparent association with the development of CAD in this study population." @default.
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• W2283346859 date "2014-01-08" @default.
• W2283346859 modified "2023-09-22" @default.
• W2283346859 title "Genetic Variants at the Apo-A1 Gene in Association with Coronary Artery Disease" @default.
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