FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2283705032> ?p ?o ?g. }

• W2283705032 endingPage "10431" @default.
• W2283705032 startingPage "10431" @default.
• W2283705032 abstract "The CRISPR-Cas system is a powerful tool for generating genetically modified animals; however, targeted knock-in (KI) via homologous recombination remains difficult in zygotes. Here we show efficient gene KI in rats by combining CRISPR-Cas with single-stranded oligodeoxynucleotides (ssODNs). First, a 1-kb ssODN co-injected with guide RNA (gRNA) and Cas9 messenger RNA produce GFP-KI at the rat Thy1 locus. Then, two gRNAs with two 80-bp ssODNs direct efficient integration of a 5.5-kb CAG-GFP vector into the Rosa26 locus via ssODN-mediated end joining. This protocol also achieves KI of a 200-kb BAC containing the human SIRPA locus, concomitantly knocking out the rat Sirpa gene. Finally, three gRNAs and two ssODNs replace 58-kb of the rat Cyp2d cluster with a 6.2-kb human CYP2D6 gene. These ssODN-mediated KI protocols can be applied to any target site with any donor vector without the need to construct homology arms, thus simplifying genome engineering in living organisms." @default.
• W2283705032 created "2016-06-24" @default.
• W2283705032 creator A5004721817 @default.
• W2283705032 creator A5031841952 @default.
• W2283705032 creator A5047033115 @default.
• W2283705032 creator A5048938769 @default.
• W2283705032 creator A5052216599 @default.
• W2283705032 creator A5065319313 @default.
• W2283705032 date "2016-01-20" @default.
• W2283705032 modified "2023-10-18" @default.
• W2283705032 title "ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes." @default.
• W2283705032 cites W1965025947 @default.
• W2283705032 cites W1967418819 @default.
• W2283705032 cites W1970280807 @default.
• W2283705032 cites W1974717031 @default.
• W2283705032 cites W1977036449 @default.
• W2283705032 cites W1998605457 @default.
• W2283705032 cites W2002764930 @default.
• W2283705032 cites W2003171404 @default.
• W2283705032 cites W2007818617 @default.
• W2283705032 cites W2010711320 @default.
• W2283705032 cites W2013916122 @default.
• W2283705032 cites W2019217866 @default.
• W2283705032 cites W2019262146 @default.
• W2283705032 cites W2032764699 @default.
• W2283705032 cites W2038659500 @default.
• W2283705032 cites W2039705224 @default.
• W2283705032 cites W2041389422 @default.
• W2283705032 cites W2052716799 @default.
• W2283705032 cites W2053745385 @default.
• W2283705032 cites W2058220225 @default.
• W2283705032 cites W2058792860 @default.
• W2283705032 cites W2064815984 @default.
• W2283705032 cites W2065395033 @default.
• W2283705032 cites W2071638544 @default.
• W2283705032 cites W2074364455 @default.
• W2283705032 cites W2075131557 @default.
• W2283705032 cites W2078846065 @default.
• W2283705032 cites W2085146326 @default.
• W2283705032 cites W2088453439 @default.
• W2283705032 cites W2089589904 @default.
• W2283705032 cites W2092002531 @default.
• W2283705032 cites W2094480378 @default.
• W2283705032 cites W2095041848 @default.
• W2283705032 cites W2095573728 @default.
• W2283705032 cites W2096261947 @default.
• W2283705032 cites W2099159538 @default.
• W2283705032 cites W2111568030 @default.
• W2283705032 cites W2116486533 @default.
• W2283705032 cites W2121633690 @default.
• W2283705032 cites W2121839178 @default.
• W2283705032 cites W2122375374 @default.
• W2283705032 cites W2122563582 @default.
• W2283705032 cites W2123366092 @default.
• W2283705032 cites W2123746851 @default.
• W2283705032 cites W2133309545 @default.
• W2283705032 cites W2139229198 @default.
• W2283705032 cites W2140754284 @default.
• W2283705032 cites W2152090841 @default.
• W2283705032 cites W2156949319 @default.
• W2283705032 cites W2163090183 @default.
• W2283705032 cites W2170039745 @default.
• W2283705032 doi "https://doi.org/10.1038/ncomms10431" @default.
• W2283705032 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4736110" @default.
• W2283705032 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26786405" @default.
• W2283705032 hasPublicationYear "2016" @default.
• W2283705032 type Work @default.
• W2283705032 sameAs 2283705032 @default.
• W2283705032 citedByCount "273" @default.
• W2283705032 countsByYear W22837050322016 @default.
• W2283705032 countsByYear W22837050322017 @default.
• W2283705032 countsByYear W22837050322018 @default.
• W2283705032 countsByYear W22837050322019 @default.
• W2283705032 countsByYear W22837050322020 @default.
• W2283705032 countsByYear W22837050322021 @default.
• W2283705032 countsByYear W22837050322022 @default.
• W2283705032 countsByYear W22837050322023 @default.
• W2283705032 crossrefType "journal-article" @default.
• W2283705032 hasAuthorship W2283705032A5004721817 @default.
• W2283705032 hasAuthorship W2283705032A5031841952 @default.
• W2283705032 hasAuthorship W2283705032A5047033115 @default.
• W2283705032 hasAuthorship W2283705032A5048938769 @default.
• W2283705032 hasAuthorship W2283705032A5052216599 @default.
• W2283705032 hasAuthorship W2283705032A5065319313 @default.
• W2283705032 hasBestOaLocation W22837050321 @default.
• W2283705032 hasConcept C104317684 @default.
• W2283705032 hasConcept C111829913 @default.
• W2283705032 hasConcept C132455925 @default.
• W2283705032 hasConcept C144501496 @default.
• W2283705032 hasConcept C153911025 @default.
• W2283705032 hasConcept C2796436 @default.
• W2283705032 hasConcept C54355233 @default.
• W2283705032 hasConcept C67705224 @default.
• W2283705032 hasConcept C70721500 @default.
• W2283705032 hasConcept C84597430 @default.
• W2283705032 hasConcept C86803240 @default.
• W2283705032 hasConcept C97702854 @default.
• W2283705032 hasConcept C98108389 @default.

• next