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- W2284096126 abstract "The effect of bubble-free oxygenation on the stability of a bi-enzymatic system with redox mediator regeneration for the conversion of lactose to lactobionic acid was investigated in a miniaturized reactor containing a composite, oxygen-permeable membrane for aeration.Earlier investigations of this biocatalytic oxidation have shown that the dispersive addition of oxygen can cause significant enzyme inactivation. In the process studied, the enzyme cellobiose dehydrogenase (CDH) oxidizes lactose at the C-1 position of the reducing sugar moiety to lactobionolactone, which spontaneously hydrolyzes to lactobionic acid. 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt was used as electron acceptor for CDH and was continuously regenerated (reoxidized) by laccase, a blue multi-copper oxidase. Oxygen served as the terminal electron acceptor of the reaction and was fully reduced to water by laccase. Conversions were conducted both in the reaction-limited and diffusion-limited regime to study catalyst inactivation. Overall, the specific productivities obtained in the membrane reactor are similar to the reaction in a conventionally aerated batch reactor, but the catalyst stability is, in comparison, significantly increased when using bubble-free oxygenation." @default.
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- W2284096126 date "2008-01-01" @default.
- W2284096126 modified "2023-09-25" @default.
- W2284096126 title "Bubble-Free Oxygenation for Enzymatic Oxidation of Lactose to Lactobionic Acid" @default.
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