Matches in SemOpenAlex for { <https://semopenalex.org/work/W2284391844> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2284391844 endingPage "61" @default.
- W2284391844 startingPage "54" @default.
- W2284391844 abstract "Aim: The aim of this study is to produce a recombinant antigen from the envelope protein of dengue virus serotype 1, as a dengue vaccine candidate. Therefore, we intend to study expression efficiency of domain III in a bacterial host. Materials and Methods: We used the following software: Megalign (sequence alignment); Modeller (protein structure prediction), and Optimizer (coding gene sequence optimization). The optimum coding sequence was synthesized and cloned in a pET21 expression vector. Stable protein expression and optimization of antigen production was performed in an Origami strain of E. coli. Results: A consensus sequence for the domain III of dengue virus serotype 1 was produced by multiple alignment of sequences and named EDIII1. Next, we predicted EDIII1 properties by using bioinformatic software. To achieve a high expression level in the bacterial host, the coding sequence of this protein was optimized for appropriate CAI and GC content. The optimized gene was synthesized and cloned at the NdeI and XhoI sites of the pET21 expression vector in an E. coli DH5α strain. In order to have efficient protein expression and the formation of disulfide bounds, we optimized expression of EDIII1 in the Origami strain. Protein purification, using Histag affinity chromatography columns was performed and the results identified by Western blot analysis. Conclusion: The results of this study showed the efficiency of a bacterial system to highly express domain III of the dengue virus envelope protein." @default.
- W2284391844 created "2016-06-24" @default.
- W2284391844 creator A5038523946 @default.
- W2284391844 creator A5040915789 @default.
- W2284391844 creator A5041267408 @default.
- W2284391844 creator A5075273556 @default.
- W2284391844 date "2012-01-01" @default.
- W2284391844 modified "2023-09-23" @default.
- W2284391844 title "RECOMBINANT EXPRESSION OF DOMAIN III OF DENGUE VIRUS ENVELOPE PROTEIN (SEROTYPE-1)" @default.
- W2284391844 cites W1549531973 @default.
- W2284391844 cites W1574082422 @default.
- W2284391844 cites W1971875148 @default.
- W2284391844 cites W1985641916 @default.
- W2284391844 cites W1985799231 @default.
- W2284391844 cites W1989946260 @default.
- W2284391844 cites W1992473920 @default.
- W2284391844 cites W2006607348 @default.
- W2284391844 cites W2009760265 @default.
- W2284391844 cites W2028680945 @default.
- W2284391844 cites W2038433611 @default.
- W2284391844 cites W2044590093 @default.
- W2284391844 cites W2068825716 @default.
- W2284391844 cites W2088522645 @default.
- W2284391844 cites W2135873691 @default.
- W2284391844 cites W2153621251 @default.
- W2284391844 cites W2158716527 @default.
- W2284391844 cites W2164191529 @default.
- W2284391844 cites W2170703458 @default.
- W2284391844 cites W2394631972 @default.
- W2284391844 hasPublicationYear "2012" @default.
- W2284391844 type Work @default.
- W2284391844 sameAs 2284391844 @default.
- W2284391844 citedByCount "0" @default.
- W2284391844 crossrefType "journal-article" @default.
- W2284391844 hasAuthorship W2284391844A5038523946 @default.
- W2284391844 hasAuthorship W2284391844A5040915789 @default.
- W2284391844 hasAuthorship W2284391844A5041267408 @default.
- W2284391844 hasAuthorship W2284391844A5075273556 @default.
- W2284391844 hasConcept C10010492 @default.
- W2284391844 hasConcept C104317684 @default.
- W2284391844 hasConcept C153911025 @default.
- W2284391844 hasConcept C159047783 @default.
- W2284391844 hasConcept C167625842 @default.
- W2284391844 hasConcept C2522874641 @default.
- W2284391844 hasConcept C2779308166 @default.
- W2284391844 hasConcept C2779635636 @default.
- W2284391844 hasConcept C33161422 @default.
- W2284391844 hasConcept C40767141 @default.
- W2284391844 hasConcept C54355233 @default.
- W2284391844 hasConcept C86803240 @default.
- W2284391844 hasConcept C91779695 @default.
- W2284391844 hasConceptScore W2284391844C10010492 @default.
- W2284391844 hasConceptScore W2284391844C104317684 @default.
- W2284391844 hasConceptScore W2284391844C153911025 @default.
- W2284391844 hasConceptScore W2284391844C159047783 @default.
- W2284391844 hasConceptScore W2284391844C167625842 @default.
- W2284391844 hasConceptScore W2284391844C2522874641 @default.
- W2284391844 hasConceptScore W2284391844C2779308166 @default.
- W2284391844 hasConceptScore W2284391844C2779635636 @default.
- W2284391844 hasConceptScore W2284391844C33161422 @default.
- W2284391844 hasConceptScore W2284391844C40767141 @default.
- W2284391844 hasConceptScore W2284391844C54355233 @default.
- W2284391844 hasConceptScore W2284391844C86803240 @default.
- W2284391844 hasConceptScore W2284391844C91779695 @default.
- W2284391844 hasIssue "11" @default.
- W2284391844 hasLocation W22843918441 @default.
- W2284391844 hasOpenAccess W2284391844 @default.
- W2284391844 hasPrimaryLocation W22843918441 @default.
- W2284391844 hasRelatedWork W1579322839 @default.
- W2284391844 hasRelatedWork W1981348191 @default.
- W2284391844 hasRelatedWork W1981504917 @default.
- W2284391844 hasRelatedWork W2005243362 @default.
- W2284391844 hasRelatedWork W2007739356 @default.
- W2284391844 hasRelatedWork W2035342539 @default.
- W2284391844 hasRelatedWork W2042347890 @default.
- W2284391844 hasRelatedWork W2046879219 @default.
- W2284391844 hasRelatedWork W2087714654 @default.
- W2284391844 hasRelatedWork W2139359203 @default.
- W2284391844 hasRelatedWork W2353853276 @default.
- W2284391844 hasRelatedWork W2379514765 @default.
- W2284391844 hasRelatedWork W2904782662 @default.
- W2284391844 hasRelatedWork W2996145878 @default.
- W2284391844 hasRelatedWork W3148202908 @default.
- W2284391844 hasRelatedWork W35567397 @default.
- W2284391844 hasRelatedWork W177994290 @default.
- W2284391844 hasRelatedWork W2253295921 @default.
- W2284391844 hasRelatedWork W2877212191 @default.
- W2284391844 hasRelatedWork W2933489007 @default.
- W2284391844 hasVolume "1" @default.
- W2284391844 isParatext "false" @default.
- W2284391844 isRetracted "false" @default.
- W2284391844 magId "2284391844" @default.
- W2284391844 workType "article" @default.