FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2284817959> ?p ?o ?g. }

• W2284817959 abstract "The analysis of oxidative stress-induced post-translational modifications remains challenging due to the chemical diversity of these modifications, the possibility of the presence of positional isomers and the low stoichiometry of the modified proteins present in a cell or tissue proteome. Alcoholic liver disease (ALD) is a multifactorial disease in which mitochondrial dysfunction and oxidative stress have been identified as being critically involved in the progression of the disease from steatosis to cirrhosis. Ethanol metabolism leads to increased levels of reactive oxygen species (ROS), glutathione depletion and lipid peroxidation. Posttranslational modification of proteins by electrophilic products of lipid peroxidation has been associated with governing redox-associated signaling mechanisms, but also as contributing to protein dysfunction leading to organelle and liver injury. In particular the prototypical α,β-unsaturated aldehyde, 4-hydroxy-2-nonenal (HNE), has been extensively studied as marker of increased oxidative stress in hepatocytes. In this study, we combined a LC-MS label-free quantification method and affinity enrichment to assess the dose-dependent insult by HNE on the proteome of rat liver mitochondria. We used a carbonyl-selective probe, the ARP probe, to label HNE-protein adducts and to perform affinity capture at the protein level. Using LC-MS to obtain protein abundance estimates, a list of protein targets was obtained with increasing concentration of HNE used in the exposure studies. In parallel, we performed affinity capture at the peptide level to acquire site-specific information. Examining the concentration-dependence of the protein modifications, we observed distinct reactivity profiles for HNE-protein adduction. Pathway analysis indicated that proteins associated with metabolic processes, including amino acid, fatty acid and glyoxylate and dicarboxylate metabolism, bile acid synthesis and TCA cycle, showed enhanced reactivity to HNE adduction. Whereas, proteins associated with oxidative phosphorylation displayed retardation toward HNE adduction. We provide a list of 31 protein targets with a total of 61 modification sites that may guide future targeted LC-MS assays to monitor disease progression and/or intervention in preclinical models of ALD and possibly other liver diseases with oxidative stress component." @default.
• W2284817959 created "2016-06-24" @default.
• W2284817959 creator A5002977923 @default.
• W2284817959 creator A5044454637 @default.
• W2284817959 date "2016-03-03" @default.
• W2284817959 modified "2023-10-05" @default.
• W2284817959 title "Label-Free Proteomics Assisted by Affinity Enrichment for Elucidating the Chemical Reactivity of the Liver Mitochondrial Proteome toward Adduction by the Lipid Electrophile 4-hydroxy-2-nonenal (HNE)" @default.
• W2284817959 cites W1502736133 @default.
• W2284817959 cites W1554963318 @default.
• W2284817959 cites W1964180160 @default.
• W2284817959 cites W1970122276 @default.
• W2284817959 cites W1971243875 @default.
• W2284817959 cites W1972709924 @default.
• W2284817959 cites W1974332498 @default.
• W2284817959 cites W1976428870 @default.
• W2284817959 cites W1978045299 @default.
• W2284817959 cites W1978896699 @default.
• W2284817959 cites W1985385891 @default.
• W2284817959 cites W1995454576 @default.
• W2284817959 cites W1999933488 @default.
• W2284817959 cites W2001296325 @default.
• W2284817959 cites W2003300391 @default.
• W2284817959 cites W2005205647 @default.
• W2284817959 cites W2011216141 @default.
• W2284817959 cites W2013714250 @default.
• W2284817959 cites W2019420272 @default.
• W2284817959 cites W2020481162 @default.
• W2284817959 cites W2031199024 @default.
• W2284817959 cites W2034713072 @default.
• W2284817959 cites W2037199116 @default.
• W2284817959 cites W2037504375 @default.
• W2284817959 cites W2043429649 @default.
• W2284817959 cites W2043951056 @default.
• W2284817959 cites W2044357091 @default.
• W2284817959 cites W2048138233 @default.
• W2284817959 cites W2048813900 @default.
• W2284817959 cites W2059714773 @default.
• W2284817959 cites W2060201737 @default.
• W2284817959 cites W2072059059 @default.
• W2284817959 cites W2080752012 @default.
• W2284817959 cites W2094254820 @default.
• W2284817959 cites W2103017472 @default.
• W2284817959 cites W2105027622 @default.
• W2284817959 cites W2111613226 @default.
• W2284817959 cites W2115820767 @default.
• W2284817959 cites W2129754968 @default.
• W2284817959 cites W2129852694 @default.
• W2284817959 cites W2138560564 @default.
• W2284817959 cites W2144321662 @default.
• W2284817959 cites W2146994504 @default.
• W2284817959 cites W2149461633 @default.
• W2284817959 cites W2158217645 @default.
• W2284817959 cites W2159482845 @default.
• W2284817959 cites W2159940458 @default.
• W2284817959 cites W26839371 @default.
• W2284817959 cites W4230280576 @default.
• W2284817959 cites W4294216483 @default.
• W2284817959 doi "https://doi.org/10.3389/fchem.2016.00002" @default.
• W2284817959 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4865762" @default.
• W2284817959 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27242993" @default.
• W2284817959 hasPublicationYear "2016" @default.
• W2284817959 type Work @default.
• W2284817959 sameAs 2284817959 @default.
• W2284817959 citedByCount "18" @default.
• W2284817959 countsByYear W22848179592016 @default.
• W2284817959 countsByYear W22848179592017 @default.
• W2284817959 countsByYear W22848179592018 @default.
• W2284817959 countsByYear W22848179592019 @default.
• W2284817959 countsByYear W22848179592020 @default.
• W2284817959 countsByYear W22848179592021 @default.
• W2284817959 countsByYear W22848179592022 @default.
• W2284817959 countsByYear W22848179592023 @default.
• W2284817959 crossrefType "journal-article" @default.
• W2284817959 hasAuthorship W2284817959A5002977923 @default.
• W2284817959 hasAuthorship W2284817959A5044454637 @default.
• W2284817959 hasBestOaLocation W22848179591 @default.
• W2284817959 hasConcept C104317684 @default.
• W2284817959 hasConcept C104397665 @default.
• W2284817959 hasConcept C181199279 @default.
• W2284817959 hasConcept C185592680 @default.
• W2284817959 hasConcept C2776151105 @default.
• W2284817959 hasConcept C2778004101 @default.
• W2284817959 hasConcept C2780829032 @default.
• W2284817959 hasConcept C28859421 @default.
• W2284817959 hasConcept C2994476132 @default.
• W2284817959 hasConcept C46111723 @default.
• W2284817959 hasConcept C48349386 @default.
• W2284817959 hasConcept C538909803 @default.
• W2284817959 hasConcept C55493867 @default.
• W2284817959 hasConcept C57600042 @default.
• W2284817959 hasConceptScore W2284817959C104317684 @default.
• W2284817959 hasConceptScore W2284817959C104397665 @default.
• W2284817959 hasConceptScore W2284817959C181199279 @default.
• W2284817959 hasConceptScore W2284817959C185592680 @default.
• W2284817959 hasConceptScore W2284817959C2776151105 @default.
• W2284817959 hasConceptScore W2284817959C2778004101 @default.
• W2284817959 hasConceptScore W2284817959C2780829032 @default.
• W2284817959 hasConceptScore W2284817959C28859421 @default.
• W2284817959 hasConceptScore W2284817959C2994476132 @default.
• W2284817959 hasConceptScore W2284817959C46111723 @default.

• next