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- W2284940210 abstract "To construct a cell-based agonists screening system for β2-adrenergic receptor (β2-AR) from chinese traditional medicines, β2-AR gene was amplified by PCR from rat genomic DNA. The PCR products were cloned into pcDNA3.1 and transfected into chinese hamster ovary (CHO) cell, which contained a cyclic AMP response element-linked enhanced green fluorescent protein reporter gene. One stable cell line was generated from the single-cell-derived colonies. It expressed β2-AR and provided green fluorescence when stimulated by ligands. Expression ofβ2-AR was monitored by RT-PCR and western blotting anti-β2-AR. The function of reporter gene system was studied by flow cytometry. This cell-based screening system was applied to the extracts of chinese traditional medicines (TCMs). Synephine which was obtained from Rutaceae generic plant, and higenamine which was obtained from Herba Asari or Radix Aconiti Lateralis Preparata were verified to haveβ2-AR agonist activity at receptor level. This assay could be applied in the pharmaceutical industry for screening thousands or indeed millions of natural compounds of TCMs forβ2-AR agonists very rapidly." @default.
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- W2284940210 date "2009-04-20" @default.
- W2284940210 modified "2023-09-26" @default.
- W2284940210 title "A Cell-based β2-adrenergic Receptor Agonist Functional Screening Assay for Chinese Traditional Medicines" @default.
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