Matches in SemOpenAlex for { <https://semopenalex.org/work/W2285128544> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2285128544 endingPage "1482" @default.
- W2285128544 startingPage "1475" @default.
- W2285128544 abstract "ATP-binding cassette protein E (ABCE1) has been annotated as an RNase L inhibitor in eukaryotes. Previous study showed that the overexpression of ABCE1 was related with the occurrence and clinical stage of lung adenocarcinoma. As an initial investigation into the novel functions of ABCE1, siRNA-expressing vectors targeting sites of the ABCE1 gene were constructed from RNAi-Ready pSIREN-DNR-DsRed-Express vector. Cultured 95-D and NCI-H446 lung carcinoma cells were transfected with the siRNA-expressing vectors using FuGENE 6 and transfection efficiency was determined by using fluorescence microscopy. The expression level of ABCE1 protein was determined by Western blot and immunofluorescence staining. Cell viability was determined by MTT, cell cycle was analysed by flow cytometry. The apoptotic rate was observed by ELISA. Fluorescence microscopy showed a satisfactory transfection efficiency which was about 42.70%. Cell viability and the growth fraction were markedly suppressed, whereas the apoptosis was significantly increased in SiRNA-95-D and SiRNA-NCI-H446 cells than controls (P＜0.05). It can be concluded that the siRNA targeting ABCE1 gene shows a dramatic inhibitory effect on RNA transcription and protein expression and a promoting effect on the apoptosis in 95-D/NCI-H446 cells, which offers a reliable base for the further in vivo experiment." @default.
- W2285128544 created "2016-06-24" @default.
- W2285128544 creator A5003036439 @default.
- W2285128544 creator A5035304239 @default.
- W2285128544 creator A5062240886 @default.
- W2285128544 creator A5074412777 @default.
- W2285128544 creator A5076849176 @default.
- W2285128544 date "2009-11-30" @default.
- W2285128544 modified "2023-09-22" @default.
- W2285128544 title "Suppression of ABCE1 Leads to Decreased Cell Proliferation and Increased Apoptosis in 95-D/NCI-H446 Lung Carcinoma Cells" @default.
- W2285128544 cites W2018158124 @default.
- W2285128544 cites W2033234643 @default.
- W2285128544 cites W2034269086 @default.
- W2285128544 cites W2037995961 @default.
- W2285128544 cites W2042079429 @default.
- W2285128544 cites W2086641174 @default.
- W2285128544 cites W2088987694 @default.
- W2285128544 cites W2093812938 @default.
- W2285128544 cites W2112053351 @default.
- W2285128544 cites W2126675331 @default.
- W2285128544 cites W2138198587 @default.
- W2285128544 cites W2143996639 @default.
- W2285128544 hasPublicationYear "2009" @default.
- W2285128544 type Work @default.
- W2285128544 sameAs 2285128544 @default.
- W2285128544 citedByCount "0" @default.
- W2285128544 crossrefType "journal-article" @default.
- W2285128544 hasAuthorship W2285128544A5003036439 @default.
- W2285128544 hasAuthorship W2285128544A5035304239 @default.
- W2285128544 hasAuthorship W2285128544A5062240886 @default.
- W2285128544 hasAuthorship W2285128544A5074412777 @default.
- W2285128544 hasAuthorship W2285128544A5076849176 @default.
- W2285128544 hasConcept C104317684 @default.
- W2285128544 hasConcept C153911025 @default.
- W2285128544 hasConcept C166703698 @default.
- W2285128544 hasConcept C190283241 @default.
- W2285128544 hasConcept C22615655 @default.
- W2285128544 hasConcept C2776415932 @default.
- W2285128544 hasConcept C2780783641 @default.
- W2285128544 hasConcept C29537977 @default.
- W2285128544 hasConcept C53227056 @default.
- W2285128544 hasConcept C54009773 @default.
- W2285128544 hasConcept C54355233 @default.
- W2285128544 hasConcept C553184892 @default.
- W2285128544 hasConcept C55493867 @default.
- W2285128544 hasConcept C62112901 @default.
- W2285128544 hasConcept C67705224 @default.
- W2285128544 hasConcept C81885089 @default.
- W2285128544 hasConcept C86803240 @default.
- W2285128544 hasConceptScore W2285128544C104317684 @default.
- W2285128544 hasConceptScore W2285128544C153911025 @default.
- W2285128544 hasConceptScore W2285128544C166703698 @default.
- W2285128544 hasConceptScore W2285128544C190283241 @default.
- W2285128544 hasConceptScore W2285128544C22615655 @default.
- W2285128544 hasConceptScore W2285128544C2776415932 @default.
- W2285128544 hasConceptScore W2285128544C2780783641 @default.
- W2285128544 hasConceptScore W2285128544C29537977 @default.
- W2285128544 hasConceptScore W2285128544C53227056 @default.
- W2285128544 hasConceptScore W2285128544C54009773 @default.
- W2285128544 hasConceptScore W2285128544C54355233 @default.
- W2285128544 hasConceptScore W2285128544C553184892 @default.
- W2285128544 hasConceptScore W2285128544C55493867 @default.
- W2285128544 hasConceptScore W2285128544C62112901 @default.
- W2285128544 hasConceptScore W2285128544C67705224 @default.
- W2285128544 hasConceptScore W2285128544C81885089 @default.
- W2285128544 hasConceptScore W2285128544C86803240 @default.
- W2285128544 hasIssue "11" @default.
- W2285128544 hasLocation W22851285441 @default.
- W2285128544 hasOpenAccess W2285128544 @default.
- W2285128544 hasPrimaryLocation W22851285441 @default.
- W2285128544 hasRelatedWork W1189139043 @default.
- W2285128544 hasRelatedWork W2000192089 @default.
- W2285128544 hasRelatedWork W2188463817 @default.
- W2285128544 hasRelatedWork W2388562155 @default.
- W2285128544 hasRelatedWork W2411713358 @default.
- W2285128544 hasRelatedWork W2413057792 @default.
- W2285128544 hasRelatedWork W2414655722 @default.
- W2285128544 hasRelatedWork W2421694109 @default.
- W2285128544 hasRelatedWork W2468968150 @default.
- W2285128544 hasRelatedWork W2607295787 @default.
- W2285128544 hasRelatedWork W2701109064 @default.
- W2285128544 hasRelatedWork W3028640417 @default.
- W2285128544 hasRelatedWork W3028883206 @default.
- W2285128544 hasRelatedWork W3029272134 @default.
- W2285128544 hasRelatedWork W3029957805 @default.
- W2285128544 hasRelatedWork W3031070862 @default.
- W2285128544 hasRelatedWork W3031474704 @default.
- W2285128544 hasRelatedWork W3032140951 @default.
- W2285128544 hasRelatedWork W3032619268 @default.
- W2285128544 hasRelatedWork W3142899340 @default.
- W2285128544 hasVolume "36" @default.
- W2285128544 isParatext "false" @default.
- W2285128544 isRetracted "false" @default.
- W2285128544 magId "2285128544" @default.
- W2285128544 workType "article" @default.