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- W2285145553 abstract "In this thesis, the physical arrangement of genes in the unc-22(ZV) region of the nematode Caenorhabditis elegans was studied using the methods of deficiency mapping and germline transformation rescue. In Part 1, I describe the isolation and characterization of 22 deficiencies affecting the unc-22 gene. These deficiencies were recovered following treatment with formaldehyde or 254-nm ultraviolet light. The extents of these deficiencies were determined by either standard complementation tests, or, in the case of two of the deficiencies, by a polymerase chain reaction based method. This analysis has resulted in the unambiguous placement of eight genes on the genetic map, and shown that of eight genes which have thus far been molecularly identified in the let-56 unc-22 interval, at least five and as many as seven are not required for viability under laboratory conditions. Analysis of the breakpoints of the 22 new deficiencies, and of 15 previously identified unc-22 deficiencies, indicate that the unc-22 let-52 interval is a hot spot for unc-22 deficiency breakpoints. In Part 2, I describe the systematic correlation of the genetic and physical maps immediately to the right of the unc-22 gene. This was accomplished by the technique of germline transformation using cosmid clones from the physical map. Three genetic loci, let-52,let-661 and let-93, were positioned on the physical map using this procedure. Analysis of these data indicates an average of one essential gene per every two cosrnids in the region immediately to the right of the unc-22 gene, and suggests that the clustering of deficiency breakpoints between unc-22 and let-52 is not due to a large physical distance separating the two loci." @default.
- W2285145553 created "2016-06-24" @default.
- W2285145553 creator A5016348509 @default.
- W2285145553 date "1994-01-01" @default.
- W2285145553 modified "2023-09-23" @default.
- W2285145553 title "Deficiency mapping and correlation of the genetic and physical maps in the unc-22(IV) region of Caenorhabditis elegans" @default.
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- W2285145553 hasPublicationYear "1994" @default.
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