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- W2285293634 abstract "Glial fibrillary acidic protein (GFAP) is the main intermediate filament (IF) in astrocytes. The GFAP gene can give rise to different splice isoforms, of which GFAPα is the canonical isoform. GFAPδ is an isoform which in the human SVZ is expressed in specific astrocytes; the adult neural stem cells. Expression differences between these isoforms lead to the hypothesis that GFAPδ may play a functional role in specific subtypes of astrocytes. In overexpression studies, we investigated the effects of GFAP isoform expression on the IF network and functional features of neurogenic astrocytes. We confirmed that GFAPδ expression collapses the GFAP network which altered cell morphology but not motility or proliferation rate. Since there is a constant exchange of proteins from an insoluble form to soluble form in the cytoplasm we assessed the dynamic properties of GFAPα and GFAPδ. The exchange rate of GFAPδ was lower compared to GFAPα and the exchange rates of both isoforms were decreased in a collapsed IF network. A role for GFAPδ in both cell motility and cell adhesion was found in experiments where the endogenous GFAP isoform levels were altered with specific isoform knockdown. Moreover, the expression levels of integrins, plectin and Laminin1a were regulated in cells with a high GFAPδ:GFAPɑ ratio. The molecular mechanisms underlying the influence of GFAPδ on cell motility, adhesion, and extracellular matrix (ECM) related protein expression is still unknown. Future research should focus on the link between the IF network, the ECM, integrin signalling and the role of GFAPδ therein." @default.
- W2285293634 created "2016-06-24" @default.
- W2285293634 creator A5022749457 @default.
- W2285293634 date "2014-01-01" @default.
- W2285293634 modified "2023-09-24" @default.
- W2285293634 title "The astrocytic cytoskeleton: Unravelling the role of GFAPδ" @default.
- W2285293634 cites W141378801 @default.
- W2285293634 cites W1504164986 @default.
- W2285293634 cites W1530407234 @default.
- W2285293634 cites W1551925364 @default.
- W2285293634 cites W1584602900 @default.
- W2285293634 cites W1755868117 @default.
- W2285293634 cites W1840213669 @default.
- W2285293634 cites W19166236 @default.
- W2285293634 cites W1927021570 @default.
- W2285293634 cites W1943067311 @default.
- W2285293634 cites W1947484002 @default.
- W2285293634 cites W1950479938 @default.
- W2285293634 cites W1965944825 @default.
- W2285293634 cites W1967265143 @default.
- W2285293634 cites W1969465898 @default.
- W2285293634 cites W1970209924 @default.
- W2285293634 cites W1972999695 @default.
- W2285293634 cites W1974396181 @default.
- W2285293634 cites W1974689368 @default.
- W2285293634 cites W1975492746 @default.
- W2285293634 cites W1976221321 @default.
- W2285293634 cites W1977329543 @default.
- W2285293634 cites W1979498042 @default.
- W2285293634 cites W1981449360 @default.
- W2285293634 cites W1981546185 @default.
- W2285293634 cites W198175277 @default.
- W2285293634 cites W1981881062 @default.
- W2285293634 cites W1983383222 @default.
- W2285293634 cites W1984971014 @default.
- W2285293634 cites W1985478809 @default.
- W2285293634 cites W1987432833 @default.
- W2285293634 cites W1988605914 @default.
- W2285293634 cites W1989696297 @default.
- W2285293634 cites W1993501781 @default.
- W2285293634 cites W1994622161 @default.
- W2285293634 cites W1994901446 @default.
- W2285293634 cites W1995513189 @default.
- W2285293634 cites W1996241541 @default.
- W2285293634 cites W1996813073 @default.
- W2285293634 cites W1998917620 @default.
- W2285293634 cites W1999495459 @default.
- W2285293634 cites W2002735784 @default.
- W2285293634 cites W2003517450 @default.
- W2285293634 cites W2004251958 @default.
- W2285293634 cites W2004912657 @default.
- W2285293634 cites W2009761723 @default.
- W2285293634 cites W2011205671 @default.
- W2285293634 cites W2012893265 @default.
- W2285293634 cites W2013540406 @default.
- W2285293634 cites W2016410251 @default.
- W2285293634 cites W2017946350 @default.
- W2285293634 cites W2019746097 @default.
- W2285293634 cites W2020942562 @default.
- W2285293634 cites W2022345246 @default.
- W2285293634 cites W2023154921 @default.
- W2285293634 cites W2023754965 @default.
- W2285293634 cites W2027119303 @default.
- W2285293634 cites W2029976952 @default.
- W2285293634 cites W2032584413 @default.
- W2285293634 cites W2033569984 @default.
- W2285293634 cites W2038935831 @default.
- W2285293634 cites W2039930606 @default.
- W2285293634 cites W2042499044 @default.
- W2285293634 cites W2042831411 @default.
- W2285293634 cites W2043420877 @default.
- W2285293634 cites W2044968967 @default.
- W2285293634 cites W2045492362 @default.
- W2285293634 cites W2045967742 @default.
- W2285293634 cites W2046548745 @default.
- W2285293634 cites W2048734728 @default.
- W2285293634 cites W2054467590 @default.
- W2285293634 cites W2057672006 @default.
- W2285293634 cites W2058277646 @default.
- W2285293634 cites W2059028264 @default.
- W2285293634 cites W2059322936 @default.
- W2285293634 cites W2059423136 @default.
- W2285293634 cites W2059688595 @default.
- W2285293634 cites W2067129601 @default.
- W2285293634 cites W2067466312 @default.
- W2285293634 cites W2067605157 @default.
- W2285293634 cites W2068514788 @default.
- W2285293634 cites W2070280831 @default.
- W2285293634 cites W2070916476 @default.
- W2285293634 cites W2071518948 @default.
- W2285293634 cites W2071802665 @default.
- W2285293634 cites W2072320390 @default.
- W2285293634 cites W2073234761 @default.
- W2285293634 cites W2073390876 @default.
- W2285293634 cites W2074661552 @default.
- W2285293634 cites W2075807595 @default.
- W2285293634 cites W2076022561 @default.
- W2285293634 cites W2076954429 @default.
- W2285293634 cites W2078525195 @default.
- W2285293634 cites W2079281464 @default.